兔坐骨神经高速弹丸震荡伤后腰段脊髓运动神经元及胶质细胞凋亡规律

发布时间：2018-08-05 16:09

【摘要】：目的　探讨坐骨神经高速弹丸震荡伤后腰段脊髓运动神经元及胶质细胞凋亡规律。方法　 6 5只大耳白兔分为正常对照组、高速弹丸震荡伤组 (震荡伤组 )和切割伤组。震荡伤组致伤靶点为兔右大腿外侧坐骨神经体表投影线中点 ,切割伤组在同一水平切断右坐骨神经。应用DNA电泳、原位末端标记法 (TUNEL)染色和流式细胞仪进行细胞凋亡定性定量检测。　结果　震荡伤组伤后 2周运动神经元数明显减少 ;伤后 1,2周 ,可见TUNEL阳性运动神经元及胶质细胞 ,DNA电泳出现凋亡梯形带 ,流式细胞仪检测可见亚二倍体峰 ;震荡伤组伤后 1,2周 ,细胞凋亡百分比分别为 10 .6 %和 2 6 .4 %。切割伤组仅在
[Abstract]:Objective to investigate the apoptosis of spinal motoneurons and glial cells in lumbar spinal cord after high speed projectile shock injury of sciatic nerve. Methods Sixty-five rabbits were divided into normal control group, high velocity projectile shock injury group and cutting injury group. The target of the shock injury group was the midpoint of the projection line of the lateral sciatic nerve in the right thigh, and the right sciatic nerve was cut off at the same level in the incised group. DNA electrophoresis, in situ end labeling (TUNEL) staining and flow cytometry were used to detect apoptosis qualitatively and quantitatively. Results in shock injury group, the number of motor neurons decreased significantly 2 weeks after injury, 1 and 2 weeks after injury. TUNEL positive motor neurons and glial cells showed apoptosis trapezoid bands, and hypodiploid peaks were detected by flow cytometry, and the percentage of apoptosis in shock injury group was 10.6% and 26.4%, respectively. Cut group only in
【作者单位】：第三军医大学附属大坪医院野战外科研究所第六研究室第三军医大学附属大坪医院野战外科研究所第六研究室第三军医大学附属大坪医院野战外科研究所第六研究室第三军医大学附属大坪医院野战外科研究所第六研究室第三军医大学附属大坪医院野战外科研究所第六研究室
【基金】：国家重点基础研究发展规划专项基金资助项目(G19990 5 42 0 6)
【分类号】：R82

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