模拟微重力对神经细胞形态及其生长的影响

发布时间：2018-08-23 13:55

【摘要】：目的探讨模拟微重力对神经细胞的形态、结构和生长的影响。方法分离新生鼠原代神经细胞,接种于Cytodex3型微载体,培养3d后转移入旋转细胞培养系统,通过Image-Pro Plus软件进行神经细胞轴突长度测量,用透射电镜观察神经细胞超微结构的变化。结果模拟微重力条件下培养5d的神经细胞的轴突长度大于正常重力条件下培养的神经细胞,两组数据相比差异显著[(65.34±8.38)μmvs.(56.10±8.57)μm,P0.01];用模拟微重力培养细胞的上清液和正常重力培养的上清液进行交换后,用模拟微重力培养上清液培养的神经细胞的轴突长度有所延长[(60.91±5.04)μmvs.(65.67±5.61)μm,P0.01;(54.65±5.49)μmvs.(60.89±6.29)μm,P0.01]。透射电镜下观察,模拟微重力条件下培养5d的神经细胞中以成熟神经元为主,核周间隙明显,胞浆其内有丰富的细胞器结构,散在有很多糖原颗粒。结论模拟微重力影响神经细胞的轴突长度及神经细胞的超微结构,同时引起了细胞培养上清液中某些成分的变化,成分变化了的上清可以继续引起神经细胞轴突长度的变化。
[Abstract]:Objective To investigate the effects of simulated microgravity on the morphology, structure and growth of neurons.Methods Primary neurons of neonatal rats were isolated and inoculated into Cytodex-3 microcarrier. After 3 days of culture, they were transferred into rotating cell culture system. The axon length of neurons was measured by image-Pro Plus software. The ultranodules of neurons were observed by transmission electron microscope. Results The axon length of neurons cultured under simulated microgravity for 5 days was longer than that of neurons cultured under normal gravity, and the difference between the two groups was significant [(65.34 The length of axons of nerve cells cultured in the supernatant of simulated microgravity medium was prolonged to a certain extent [(60.91.04) microns vs. (65.67.61) microns, P 0.01; (54.65.49) microns vs. (60.89.29) microns, P 0.01]. Conclusion Simulated microgravity can affect the length of axons and the ultrastructure of nerve cells, and cause the changes of some components in the supernatant of cell culture. The supernatant with the changes of components can continue to cause the changes of axon length of nerve cells.
【作者单位】：哈尔滨医科大学神经生物学教研室哈尔滨医科大学神经生物学教研室哈尔滨医科大学神经生物学教研室哈尔滨医科大学神经生物学教研室哈尔滨医科大学神经生物学教研室哈尔滨医科大学神经生物学教研室哈尔滨医科大学神经生物学教研室哈尔滨医科大学神经生物学教研室
【基金】：黑龙江省卫生厅科技攻关课题(2005-15) 哈尔滨医科大学青年科学基金(060038)
【分类号】：R852.22

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