豚鼠耳石修复再生的超微结构及元素成分动态变化

发布时间：2018-08-24 12:45

【摘要】：目的观察成熟耳石损伤后修复再生的形态学和元素变化特点,为深入认识其修复再生的规律和机理提供依据。方法给予过载刺激(10 Gy 5min)造成18只豚鼠耳石的损伤破坏,用扫描电镜(scanning electron microscopy,SEM)分别观察过载刺激后1h、2d、8d时其椭圆囊囊斑,用X射线显微能谱分析技术(electron probe X-ray microanalysis,EPMA)测量不同形态类别的耳石元素构成。结果过载刺激后1h正常耳石形态消失,被大量球状物代替,2d时球状物逐渐矿化,8d时哑铃状耳石出现并与矿化球状物共存,此外各时期均可见各种耳石的变性。EPMA结果显示,变性耳石Ca元素含量显著增高和P元素含量显著降低。另外,球状物在由初期向成熟矿化期转化过程中,表现出P元素含量逐渐增加和Ca元素含量逐渐减少的趋势,但未达到统计学显著水平。结论成熟耳石损伤破坏后可以修复再生,该过程中形态学的变化伴随着元素构成的变化。
[Abstract]:Objective To observe the morphological and elemental changes in the repair and regeneration of mature otolith and to provide evidence for further understanding of the regularity and mechanism of the repair and regeneration of mature otolith.Methods 18 guinea pigs were injured and damaged by overload stimulation (10 Gy 5 min). Results One hour after stimulation, the normal otolith disappeared and was replaced by a large number of spheres. The spheres gradually mineralized on the second day, and dumbbell-shaped otoliths appeared and coexisted with mineralized spheres on the eighth day. The results of EPMA showed that the contents of Ca and P in the denatured otoliths increased significantly and decreased significantly. In addition, the contents of P and Ca in the spheres increased gradually from the initial stage to the mature mineralization stage, but did not reach the statistical significant level. Conclusion Mature otolith can be repaired and regenerated after damage. Morphological changes are accompanied by changes in element composition.
【作者单位】：空军航空医学研究所空军航空医学研究所空军航空医学研究所空军航空医学研究所空军航空医学研究所空军航空医学研究所
【基金】：空军后勤部十五规划项目资助(KH0108014)
【分类号】：R852.3

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