晕船适应与不适应大鼠脑干蛋白质双向电泳图谱的比较与鉴定

发布时间：2018-08-28 11:05

【摘要】：目的:建立晕船适应及不适应大鼠脑干总蛋白质的双向电泳图谱,鉴定差异表达蛋白,探讨晕船适应的可能机制。方法:30只大鼠根据模拟晕船刺激后异嗜高岭土量的增减分为不晕组(n=10)、晕船适应组(n=12)和不适应组(n=8),另设未接受模拟晕船刺激的正常大鼠作为空白对照组(n=10)。应用双向电泳技术,分离晕船适应组和不适应组大鼠脑干蛋白(n=6),肽指纹图谱鉴定两组间差异表达蛋白质。结果:模拟晕船刺激21 d后,与空白对照组相比,晕船适应组大鼠异嗜高岭土量经历了一个由少到多又逐渐回落的过程,不适应组大鼠异嗜高岭土量则始终维持在一个较高的水平(P0.01或P0.05)。获得了9个晕船适应相关蛋白质:
[Abstract]:Aim: to establish a two dimensional electrophoretic map of brain stem total protein in seasickness adaptation and unadapted rats, to identify differentially expressed proteins and to explore the possible mechanism of seasickness adaptation. Methods according to the increase or decrease of isophilic kaolin content after simulated seasickness stimulation, 30 rats were divided into three groups: no dizzy group (n = 10), seasickness adaptation group (n = 12) and inadaptation group (n = 8), and normal rats without simulated seasickness stimulation were used as blank control group (n = 10). Brain stem protein (nong6) was isolated from seasickness adaptation group and unadapted group by two dimensional electrophoretic technique, and the differentially expressed proteins were identified by peptide fingerprinting. Results: after 21 days of simulated seasickness stimulation, compared with the control group, the amount of isophoric kaolin in the seasickness adaptation group experienced a process from less to more and gradually decreased. In the unadapted group, the amount of isophoric kaolin remained at a high level (P0.01 or P0.05). Nine seasickness related proteins were obtained:
【作者单位】：第二军医大学海军医学系军队卫生学教研室第二军医大学基础医学部生物化学与分子生物学教研室第二军医大学基础医学部生物化学与分子生物学教研室第二军医大学海军医学系军队卫生学教研室第二军医大学基础医学部生物化学与分子生物学教研室第二军医大学海军医学系军队卫生学教研室
【基金】：军事医学专项基金(2001J-4).~~
【分类号】：R83

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