空间飞行条件下心肌细胞发生功能减退与微管解聚

发布时间：2018-09-10 17:07

【摘要】：空间飞行对心血管系统具有深远的影响.但是,目前关于心肌细胞如何对空间飞行条件发生响应尚未见报道.本研究报道了空间飞行对体外培养的心肌细胞结构与功能的影响.神舟六号飞船将原代培养的新生大鼠心肌细胞载入太空,并于发射后4h进行在轨激活.其中8个样品分别于发射后4,48以及96h进行在轨固定,并于飞船返回后进行细胞骨架的荧光染色观察;另外2个样品未进行固定,于飞船返回后进行心肌细胞收缩及分泌功能的分析;地面样品在实验室中进行平行处理.飞行115h结束后,与地面样品比较,飞行样品中心肌细胞的自发搏动位点显著减少,同时搏动位点中的细胞收缩频率明显加快,并失去同步性;对飞行样品培养液进行的放射免疫检测显示,飞行细胞的心钠素分泌水平下降59.6%.对固定样品进行的激光共聚焦显微图像分析显示,飞行细胞呈现时间依赖性的微管解聚,而微丝骨架的结构与分布没有明显变化.总之,上述结果提示,空间飞行诱发体外培养的心肌细胞发生功能减退和微管解聚,对于进一步研究空间心血管功能紊乱的机制提供了细胞学基础.
[Abstract]:Space flight has a profound impact on the cardiovascular system. However, the response of cardiac myocytes to space flight conditions has not been reported. In this study, the effects of space flight on the structure and function of cultured cardiomyocytes were reported. Shenzhou-6 spacecraft loaded primary cultured neonatal rat cardiomyocytes into space and was activated in orbit 4 hours after launch. Eight of the samples were fixed in orbit at 48 and 96 hours after launch, and the cytoskeleton was observed by fluorescence staining on the return of the spacecraft, while the other two samples were not fixed. The contractile and secretory function of cardiomyocytes was analyzed after the return of the spaceship, and the ground samples were treated parallel in the laboratory. At the end of 115 h flight, compared with the ground samples, the spontaneous pulsatile sites of the cardiac myocytes in the flight samples decreased significantly, and the contraction frequency of the cells in the pulsatile sites increased significantly and lost synchronicity. Radioimmunoassay showed that atrial natriuretic peptide (ANP) secretion of flying cells decreased 59.6%. The laser confocal microscopic image analysis of the fixed samples showed that the flying cells showed time-dependent microtubule depolymerization, while the structure and distribution of the microfilament skeleton did not change significantly. In conclusion, these results suggest that space flight induces hypofunction and microtubule depolymerization of cultured cardiomyocytes, which provides a cytological basis for further study of the mechanism of spatial cardiovascular dysfunction.
【作者单位】：中国航天员科研训练中心航天细胞分子生物学实验室中国航天员科研训练中心航天细胞分子生物学实验室中国航天员科研训练中心航天细胞分子生物学实验室中国航天员科研训练中心航天细胞分子生物学实验室中国航天员科研训练中心航天细胞分子生物学实验室中国航天员科研训练中心航天细胞分子生物学实验室中国航天员科研训练中心航天细胞分子生物学实验室中国航天员科研训练中心航天细胞分子生物学实验室中国航天员科研训练中心航天细胞分子生物学实验室中国航天员科研训练中心航天细胞分子生物学实验室中国航天员科研训练中心航天细胞分子生物学实验室中国航天员科研训练中心航天细胞分子生物学实验室
【基金】：中国载人航天工程、国家高技术研究发展计划(批准号:2002AA743051) 国家重点基础研究发展计划(批准号:2006CB705704) 国家自然科学基金(批准号:30370365) 中国航天医学工程预先研究项目(批准号:SJ200508)资助项目
【分类号】：R85

【参考文献】