高温复合创伤对兔血一氧化氮及平均动脉压的影响

发布时间：2018-10-11 18:15

【摘要】：目的　探讨急性热暴露复合创伤后血浆一氧化氮 (NO)早期变化及其对血压的可能影响 ,为急性应激条件下NO的作用机制研究提供理论依据。方法　建立兔急性热暴露复合创伤模型 ,实验动物分为常温无创组、常温创伤组、高温无创组、高温创伤组。于 0、1、2h不同时间点取血测定NO含量 ,全程监测血压变化。结果　急性热暴露创伤后 1h左右 ,兔血浆NO浓度下降 ,然后升高 ,高温创伤组NO浓度在 1、2h时分别为 (42 .75± 8.2 4)、(5 9.5 4± 9.0 5 ) μmol/L ,差异有显著性 (P 0 .0 5 ) ,而正常对照组则为 (5 6 .6 3± 3.79)、(5 5 .2 2± 7.15 ) μmol/L ,两组于 1h时的差异有显著性 (P 0 .0 5 )。在高温和创伤条件下 ,兔平均动脉压 (MAP)于 6 0～ 70min降至最低点 ,继之有一个短暂的回升 ,然后则迅速下降。结论　急性热暴露复合创伤兔早期血浆NO浓度先降低后升高 ,且与MAP的变化有一定关系。
[Abstract]:Objective to investigate the early changes of plasma nitric oxide (NO) and its possible effect on blood pressure after acute heat exposure combined trauma, and to provide a theoretical basis for the study of the mechanism of NO under acute stress. Methods the rabbit model of acute heat exposure combined trauma was established. The experimental animals were divided into normal temperature noninvasive group, normal temperature trauma group, high temperature noninvasive group and high temperature trauma group. Blood samples were collected at different time points to determine the NO content and blood pressure was monitored at 0 ~ 1h for 2 h. Results the concentration of plasma NO decreased and then increased about 1 h after acute heat exposure. The concentration of NO in hyperthermia trauma group was (42.75 卤8.24), () 59.54 卤9.05 渭 mol/L (P < 0.05). The normal control group was (56.63 卤3.79), (55.22 卤7.15) 渭 mol/L, and there was significant difference between the two groups at 1 h (P 0.05). Under the condition of high temperature and trauma, the mean arterial pressure (MAP) of rabbits decreased from 60 to 70min to the lowest point, followed by a brief rise and then a rapid decline. Conclusion in the early stage of acute heat exposure combined trauma, plasma NO concentration decreased first and then increased, and was related to the change of MAP.
【作者单位】：第一军医大学高温医学研究室第一军医大学高温医学研究室山东省莱阳市第二人民医院心内科南方医院护理部第一军医大学高温医学研究室
【基金】：军队“十五”指令性课题资助项目 ( 0 1L0 5 1)
【分类号】：R82

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