神经节苷脂对急性低氧大鼠脑皮层神经元的保护作用研究

发布时间：2018-10-24 12:36

【摘要】：目的观察外源性神经节苷脂对急性减压低氧大鼠脑神经元的保护作用 ,为临床应用提供依据。方法 1 5只Wistar大鼠随机分为 :低氧组A、低氧组B与常氧组。低氧组A置于海拔 70 0 0m ,持续 5h ,低氧组B在低氧 3d前腹腔注射神经节苷脂 ( 1 0 0mg/kg/d) ,其余与低氧组A相同。电镜观察各组脑皮层神经元的超微结构改变。结果低氧组B动物的脑神经元胞体、细胞骨架和突触结构损伤的程度明显轻于低氧组A。结论神经节苷脂对低氧性脑损伤有一定的保护作用 ,这可能与其减少低氧时胞内Ca2 +蓄积和稳定CaM水平与CaMKinaseⅡ活性变化有关。
[Abstract]:Objective to observe the protective effect of exogenous ganglioside on brain neurons in rats with acute decompression and hypoxia, and to provide evidence for clinical application. Methods Fifteen Wistar rats were randomly divided into hypoxia group, hypoxia group B and normoxic group. Hypoxia group A was placed at 7 000 m above sea level for 5 hours. Rats in hypoxia group B were injected with ganglioside (10 0mg/kg/d) 3 days before hypoxia, and the rest were the same as those in hypoxia group A. Ultrastructural changes of cortical neurons in each group were observed by electron microscope. Results the degree of damage to the neuronal cell body, cytoskeleton and synaptic structure in hypoxic group B was significantly less than that in hypoxic group A. Conclusion ganglioside has a protective effect on hypoxic brain injury, which may be related to the decrease of intracellular Ca2 accumulation and stable CaM level and the change of CaMKinase 鈪，

本文编号：2291441