高温高湿环境犬肢体火器伤后TNF-α与HSP70含量变化的实验研究

发布时间：2018-11-02 12:05

【摘要】：为探讨高温高湿环境下肢体火器伤后肿瘤坏死因子 α(TNF α)与热休克蛋白 70 (HSP70 )含量变化的规律 ,将犬随机分为常温常湿组、热适应组、高温高湿组 ,分别在致伤前和火器伤后 1、3、4、6、8、10、14、18、2 4h检测TNF α与HSP70含量。结果发现 ,①伤道骨骼肌中TNF α较血浆中升高明显。常温常湿组 4h开始升高 ,14h达高峰 ;高温高湿组 1h升高 ,10h达到高峰 ,其峰值显著高于常温常湿组(P 0 0 5 ) ;热适应组变化基本和高温高湿组一致 ,只是升高幅度较低 (P0 0 5 )。②淋巴细胞中HSP70含量在高温高湿组有两个高峰 ,分别为伤后 4h和 14～ 18h ;热适应组 3h达到高峰 ,持续高峰至 6h ;常温常湿组 6h达到高峰 ,且其伤道骨骼肌HSP70含量在伤后 14～ 18h也出现一个增幅不明显的高峰期。说明高温高湿环境下肢体火器伤后TNF α与HSP70含量增高 ,且有其独特的规律
[Abstract]:In order to investigate the changes of tumor necrosis factor 伪 (TNF 伪) and heat shock protein 70 (HSP70) in firearm injured limbs under high temperature and high humidity, dogs were randomly divided into four groups: normal temperature and humidity group, heat adaptation group and high temperature and high humidity group. The contents of TNF 伪 and HSP70 were detected before injury and 1824 hours after firearm injury. The results showed that 1 TNF 伪 in skeletal muscle was significantly higher than that in plasma. In the normal temperature and humidity group, the peak value began to rise at 4 h and reached the peak at 14 h, and the peak value increased at 1 h and reached the peak at 10 h in the high temperature and high humidity group, which was significantly higher than that in the normal temperature and normal humidity group (P 0 05). The change of HSP70 in heat adaptation group was basically the same as that in high temperature and high humidity group, but the increase was lower (P0 05). 2 there were two peaks of HSP70 content in hyperthermia and high humidity group, which were 4 h and 14 ~ 18 h after injury, respectively. The heat adaptation group reached the peak at 3 h and lasted to 6 h, and the normal temperature and humidity group reached the peak at 6 h, and the HSP70 content of skeletal muscle in the injured tract also showed a peak of no significant increase at 14 ~ 18 h after injury. The results show that the content of TNF 伪 and HSP70 increases after firearm injury in high temperature and high humidity environment, and has its unique regularity.
【作者单位】：第一军医大学南方医院第一军医大学南方医院第一军医大学南方医院第一军医大学南方医院
【基金】：全军“九五”医药卫生杰出中青年基金资助课题 (编号 98J0 0 4 )
【分类号】：R826.68

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