被动运动对抗尾吊大鼠软骨退化的效果

发布时间：2018-11-16 12:48

【摘要】：目的探寻后肢去负荷过程中,能够阻止软骨退化的对抗措施。方法 SD大鼠分3组(每组8只):对照组(Con)、尾吊组(TS)、尾吊+被动运动组(TSP)。尾吊21天期间,TSP组接受由训练装置提供的被动运动训练。利用μCT与组织切片检测大鼠股骨远端软骨的变化。结果μCT结果表明,TS与TSP组大鼠股骨远端的关节软骨厚度与Con比明显下降;TS的软骨体积较Con与TSP显著下降,而Con与TSP间没有显著差异;TS与TSP的软骨的衰减系数较Con明显升高。组织切片表明,TS与TSP的软骨厚度较Con明显下降,番红O—固绿染色显示TS与TSP的基质染色程度较Con下降,软骨细胞出现减少的现象。此外,μCT所测厚度与组织切片所测厚度高度相关。结论大鼠尾吊会导致关节软骨退化,然而被动运动不能有效的阻止尾吊引起的软骨退化。
[Abstract]:Objective to explore the countermeasures to prevent cartilage degeneration during deloading of hind limbs. Methods SD rats were divided into 3 groups (8 rats in each group): control group, (Con), tail suspension group, (TS), tail suspension passive movement group, (TSP). During 21 days of tail suspension, the TSP group received passive exercise training provided by the training device. The changes of distal femur cartilage in rats were detected by 渭 CT and tissue sections. Results the results of 渭 CT showed that the thickness of articular cartilage and the ratio of Con to Con in the distal femur of TS and TSP rats were significantly decreased, the cartilage volume of TS was significantly lower than that of Con and TSP, but there was no significant difference between Con and TSP. The attenuation coefficient of TS and TSP cartilage was significantly higher than that of Con. Tissue sections showed that the thickness of cartilage in TS and TSP was significantly lower than that in Con, and the degree of matrix staining in TS and TSP was lower than that in Con, and the number of chondrocytes was decreased. In addition, the thickness measured by 渭 CT was highly correlated with the thickness measured in tissue sections. Conclusion tail suspension can cause articular cartilage degeneration in rats, but passive motion can not effectively prevent cartilage degeneration caused by tail suspension.
【作者单位】：北京航空航天大学生物与医学工程学院生物力学与力生物学教育部重点实验室;
【基金】：国家自然科学基金资助项目(31170897)
【分类号】：R852.22

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