模拟400m氦氧饱和潜水对大鼠抗氧化能力的影响

发布时间：2018-11-18 15:12

【摘要】：[目的]观察模拟400 m氦氧饱和潜水对大鼠抗氧化能力的影响。[方法]将大鼠在加压舱4.1 MPa氦氧环境中饱和暴露24 h,以常压和4.1 MPa常氧高氦环境作为对照。出舱后测定大鼠肝、脑和肺中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)活性和还原型谷胱甘肽(GSH)、丙二醛(MDA)及8-羟基脱氧鸟苷(8-OhdG)含量。[结果]4.1 MPa氦氧饱和暴露后大鼠脑SOD活性和肝、脑和肺GSH-Px活性降低(P0.05),脑和肺GSH含量减少(P0.05)。4.1 MPa常氧高氦暴露后大鼠各指标未见明显改变(P0.05)。各组MDA和8-OhdG含量差异无统计学意义(P0.05)。[结论]模拟400 m氦氧饱和潜水24 h使大鼠产生了氧化应激反应,抗氧化能力降低,其原因可能与该环境中的高分压氧有关。
[Abstract]:[objective] to observe the effect of simulated 400 m helium oxygen saturation diving on anti-oxidation ability of rats. [methods] the rats were saturated for 24 hours in a pressure chamber of 4.1 MPa helium-oxygen environment. The rats were exposed to atmospheric pressure and 4.1 MPa normoxic high-helium environment as control. The activity of superoxide dismutase (SOD),) glutathione peroxidase (GSH-px) and the contents of glutathione (GSH), malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OhdG) in rat liver, brain and lung were measured. [results] the activities of SOD and GSH-Px in liver, brain and lung were decreased after exposure to helium-oxygen saturation for 4.1 MPa in rats (P0.05). The contents of GSH in brain and lung were decreased (P0.05). There was no significant change in all indexes after exposure to normoxic high helium for 4.1 MPa (P0.05). There was no significant difference in the content of MDA and 8-OhdG in each group (P0.05). [conclusion] simulated 400 m helium-oxygen saturation diving for 24 h resulted in oxidative stress reaction and decreased antioxidant capacity in rats, which may be related to the high partial pressure oxygen in the environment.
【作者单位】：海军医学研究所;同济大学体育部;
【基金】：全军后勤科研项目(编号:AHJ08J003,AHJ11J004,CWS11J186)
【分类号】：R84

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