模拟失重大鼠脑血管周围肽能神经支配的可塑性变化

发布时间：2018-11-22 12:35

【摘要】：目的　探讨模拟失重是否可引起动脉血管周围神经支配发生可塑性变化。　方法　以尾部悬吊大鼠模型模拟失重时的血液动力学影响。以免疫组织化学 (ABC- GDN)方法 ,观察对照 (CON)、尾部悬吊 4周 (SUS- 4)和恢复 1周 (REC- 1)大鼠脑血管周围肽能神经的变化。　结果　与 CON组比较 ,脑动脉上述神经在 SU S- 4组变得更清晰、染色更深 ,而在 REC- 1组纤维变得模糊且不连续。定量观察表明 ,上述几种神经纤维的密度在 SUS- 4组显著升高 ,以大脑前动脉为例 ,含 NPY、含 CGRP及含 SP神经纤维的密度较 CON组分别升高 70 %、78%及 111%(P 0 .0 1) ;而 REC- 1组各段血管 ,则有降低的趋势 ,但多未达到显著程度。　结论　脑动脉周围肽能神经对模拟失重引起的血液动力学改变颇为敏感 ,可发生支配增强的适应性变化 ;解除后 ,则先经历支配减弱阶段 ,再恢复至正常水平。
[Abstract]:Objective to investigate whether simulated weightlessness can induce the plasticity of arterial peripheral nerve innervation. Methods the hemodynamic effects of weightlessness were simulated in tail suspension rat model. Immunohistochemical method (ABC- GDN) was used to observe the changes of cerebrovascular peripheral peptidergic nerves in (CON), rats after 4 weeks of tail suspension (SUS- 4) and 1 week of recovery (REC- 1). The results were compared with those of CON group. These nerves became clearer and more stained in SU S- 4 group, but blurred and discontinuous in REC- 1 group. Quantitative observation showed that the density of these nerve fibers increased significantly in SUS- 4 group. The density of NPY, containing CGRP and SP nerve fibers was 70% higher than that of CON group, taking the anterior cerebral artery as an example. 78% and 111% (P 0.01); However, the blood vessels in group REC- 1 had a decreasing trend, but most of them were not significant. Conclusion the peripheral peptidergic nerve of cerebral artery is sensitive to hemodynamic changes induced by simulated weightlessness. Adaptive change of dominating enhancement occurs; After the release, first experienced the dominant weakening stage, and then returned to the normal level.
【作者单位】：第四军医大学航空航天生理学教研室!组织学胚胎学教研室西安710032 第四军医大学航空航天生理学教研室!西安710032 第四军医大学航空航天生理学教研室!西安710032 第四军医大学组织学胚胎学教研室!西安710032 第四军医大学组织学胚胎学教研室!西安710032
【基金】：国家自然科学基金!( 3 93 80 0 2 1)
【分类号】：R852.22

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