不同气候地区武警士兵热休克蛋白71表达的差异性分析
发布时间：2018-12-11 21:02
【摘要】：目的 分析来自北京、郑州和广州三个地区武警士兵外周血淋巴细胞热休克蛋白 71(HSP71)基础和热应激后表达水平的差异性。方法 用流式细胞术检测淋巴细胞HSP71水平 ,用彗星试验检测淋巴细胞在基础和应激状态下DNA的损伤情况。结果 彗星试验显示 ,淋巴细胞在基础和热应激条件下 (41℃ ,1h)未发现有明显的DNA损伤 ,三个地区之间士兵淋巴细胞在基础和应激条件下DNA损伤的差异均无显著性 (P 0 .0 5 ) ;热应激后 ,三个地区士兵淋巴细胞HSP71均明显增高 ,差异有显著性 (P 0 .0 5 ) ,不同地区比较发现 ,北京士兵HSP71基础和热应激水平 (平均荧光强度分别为 84 5 .87± 135 .6 0、12 5 4 .4 7± 2 39.0 5 )均比广州士兵 (平均荧光强度分别为 70 2 .73± 184 .70、86 1.72±2 2 5 .12 )明显升高 ,且差异有显著性 (P 0 .0 5 )。结论 不同气候地区武警士兵淋巴细胞HSP71基础和热应激水平存在差异性 ,提示可以将HSP71基础和热应激水平作为评价不同气候地区武警战士在热环境下耐受能力的指标
[Abstract]:Objective to analyze the differences of heat shock protein 71 (HSP71) expression levels in peripheral blood lymphocytes of armed police soldiers from Beijing, Zhengzhou and Guangzhou. Methods HSP71 levels in lymphocytes were detected by flow cytometry and DNA damage was detected by comet assay. Results Comet assay showed that there was no obvious DNA damage in lymphocytes under basic and heat stress conditions (41 鈩，
本文编号：2373217
[Abstract]:Objective to analyze the differences of heat shock protein 71 (HSP71) expression levels in peripheral blood lymphocytes of armed police soldiers from Beijing, Zhengzhou and Guangzhou. Methods HSP71 levels in lymphocytes were detected by flow cytometry and DNA damage was detected by comet assay. Results Comet assay showed that there was no obvious DNA damage in lymphocytes under basic and heat stress conditions (41 鈩，
本文编号：2373217
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