生姜透皮贴对运动病大鼠脑组织中三种酶活性的影响

发布时间：2019-02-13 13:50

【摘要】：目的通过建立运动病模型,检测生姜透皮贴对运动病大鼠脑组织中碱性磷酸酶(AOP)、Na+-K+-ATP酶和细胞色素氧化酶(CCO)活性的变化,并探讨其治疗运动病的机制。方法雄性SD大鼠40只,随机分5组,每组8只,每天按实验方法刺激,同时实验组给予生姜透皮贴治疗。实验第3天刺激结束后迅速断头处死,冰上迅速分离脑组织,取大脑皮质和小脑皮质、脑干前庭区。采用组织化学定位方法和图像定量分析方法 ,检测各组大鼠上述三部位脑组织中ALP、Na+-K+-ATP酶及CCO活性。结果运动病大鼠三部位脑组织中ALP活性显著升高、Na+-K+-ATP酶及CCO活性明显降低。与模型组相比,使用生姜透皮贴后运动病大鼠三部位脑组织中ALP活性显著减低、Na+-K+-ATP酶及CCO活性明显升高。结论生姜透皮贴可能通过降低脑血管紧张度,改善脑血流的分布,增加脑血流量,调整脑细胞内CCO、ALP、Na+-K+-ATP酶等酶的活性,增强脑细胞的兴奋性,从而保持中枢神经系统的兴奋性,起到抗运动病的作用。
[Abstract]:Objective to investigate the changes of alkaline phosphatase (AOP), Na) -K ATP enzyme and cytochrome oxidase (CCO) activity in brain tissue of rats with motor disease by establishing a model of motor disease. Methods 40 male SD rats were randomly divided into 5 groups with 8 rats in each group. On the third day of the experiment, the rats were killed quickly after stimulation, and the cerebral cortex and cerebellar cortex were quickly separated from the ice, and the vestibular area of the brain stem was taken. Histochemical localization and image quantitative analysis were used to detect the activities of ALP,Na-K-ATP and CCO in the brain tissues of each group. Results the activity of ALP, Na-K-ATP and CCO in brain tissue of rats with motor disease were significantly increased, and the activities of Na-K-ATP and CCO were significantly decreased. Compared with the model group, the activity of ALP, Na-K-ATP and CCO in the brain tissue of rats with motor disease were significantly decreased, and the activities of Na-K-ATP and CCO were significantly increased after Ginger transdermal application. Conclusion Ginger transdermal patch may improve the distribution of cerebral blood flow, increase cerebral blood flow, regulate the activity of CCO,ALP,Na-K-ATP enzyme in brain cells, and enhance the excitability of brain cells by decreasing cerebral vascular tension, improving the distribution of cerebral blood flow, and regulating the activity of CCO,ALP,Na-K-ATP enzyme in brain cells. So as to maintain the excitability of the central nervous system, play the role of anti-motor disease.
【作者单位】：湖北职业技术学院护理学院;华中科技大学生命科学与技术学院;
【分类号】：R85

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