聚合酶链反应扩增指纹多态性快速分型技术在部队结核病分子流行病学研究中的应用

发布时间：2019-02-16 00:16

【摘要】：目的　探讨南方部队结核病的分子流行病学规律。方法　设计一对特异性IS6 110外向性引物 ,应用聚合酶链反应 (PCR)建立检测结核分支杆菌DNA指纹多态性的方法 ,分析结核分支杆菌DNA多态性与流行病学的关系。结果　共检测分析了 15 4株结核分支杆菌DNA的指纹多态性。根据这些菌株的指纹多态性特征共分为 8个类型 ,以Ⅰ型 (36 .4 % )、Ⅱ型 (31.8% )和Ⅲ型(2 1.4 % )为主 ,其余各型均少于 4 %。以 2 0～ 2 9岁和 30～ 39岁组在这三型中所占比例最大 ,分别为31.8%和 2 7.9%。驻城镇部队与驻乡村部队以及结核病患者有无卡介苗接种史 ,在这三型的
[Abstract]:Objective to investigate the molecular epidemiology of tuberculosis in southern army. Methods A pair of specific IS6 110 extroversion primers were designed to detect the DNA fingerprinting polymorphism of Mycobacterium tuberculosis by polymerase chain reaction (PCR). The relationship between DNA polymorphism and epidemiology of Mycobacterium tuberculosis was analyzed. Results the fingerprinting polymorphism of 154 strains of Mycobacterium tuberculosis DNA was analyzed. According to the fingerprinting polymorphism of these strains, they were divided into 8 types, mainly type 鈪，

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