正加速度和噪声复合应激对大鼠学习记忆的影响
发布时间：2019-03-26 10:52
【摘要】：现代战争由于其特殊性，对参战双方部分人员的精神和心理状态有着一定的损害作用和后遗影响。应激刺激的形式很多，恐惧、疼痛、缺氧、高温、寒冷、加速度、噪声等都是应激源。在军事医学领域中，应激对部队作战人员的不利影响日益凸现，并逐渐引起军事医学工作者的高度重视。 由于军事飞行特殊的工作环境，战斗机飞行员不可避免地暴露于各种应激源，其中最常见的主要有正加速度(+Gz)、噪声、振动和高温等，，已成为威胁飞行安全的重要因素。现代高性能战斗机具有很高的机动性能，其在飞行中产生的正加速度可高达+gGz，持续时间可达15～45s，并可反复出现，已超出了人体正常的耐受限度。学习与记忆是动物和人类赖以生存所不可或缺的重要脑功能，可以反映神经系统的功能状态。以往的研究工作已证实，高G值持续性正加速度暴露可引起动物脑损伤和学习记忆功能障碍，一定强度的噪声可引起人和动物学习记忆能力降低。然而，有关正加速度和噪声复合应激因素对脑的影响与防护措施尚未见研究报道。因此，阐明上述复合应激因素作用后飞行人员工作能力的变化规律，对进一步做好飞行卫生保障工作具有重要的现实意义。本研究观察 第四军医大学硕士学位论文 了正加速度和噪声复合应激对大鼠学习记忆功能的影响，旨在揭示 正加速度和噪声复合应激后大鼠学习记忆功能的变化规律，并进一 步探讨低G锻炼是否对正加速度和噪声复合应激造成的学习记忆 功能障碍具有保护作用。 本研究选用我校动物实验中心提供的雄性SD大鼠，在动物离 心机上进行招z暴露，同时利用白噪声发生器产生噪声。通过Y- 型迷宫实验、避暗实验、旷场分析实验，分别在复合应激暴露后的 即刻、2d、4d和6d，观察不同正加速度和噪声复合应激条件下大 鼠学习和记忆功能的变化情况。 本研究的主要结果及发现如下: 1.高+Gz和噪声复合应激可引起大鼠持续性学习记忆功能障 碍本实验观察了+10Gz/3min和godB(A)/3 omin噪声复合应激对 大鼠学习记忆功能的影响。结果发现，复合应激组大鼠正确率在暴 露后各时间点较对照组和噪声组均显著降低(P0.01)，反应时均 显著延长(P0.01);中央格停留时间在暴露后即刻较对照组和噪 声组显著延长(P0.01);总时间和错误数在暴露后即刻较对照组 显著增加(P0.01)，潜伏期在暴露后即刻显著缩短(P0.01)，在 暴露后6d时仍较对照组显著缩短(P0.01)。而与+l OGz组相比， 复合应激组大鼠正确率在暴露后即刻显著降低(P0.05)，反应时 显著延长(P0.01)。结果提示，+10Gz/3min和90dB(A)/30min 噪声复合应激可引起大鼠持续性学习记忆功能障碍，两种因素具有 协同效应，其中+Gz为主要影响因素。 2.短时重复高+Gz暴露复合噪声应激可引起大鼠持续性学习 记忆功能障碍本实验观察了重复5次+l 002/455暴露复合 90dB(A)/30min噪声应激对大鼠学习记忆功能的影响。结果发现， 复合应激组大鼠正确率在暴露后各时间点较对照组均显著降低(P 0.01)，反应时均显著延长(P0.01):总时间和错误数在暴露后 即刻显著增加(P0.01)。与+Gz重复暴露组相比，复合应激组大 鼠正确率在暴露后各时间点均显著降低(P0.01)，反应时在暴露 第四军医大学硕士学位论文 后4d及6d显著延长(P0.01);总时间和错误数在暴露后即刻显 著增加(P0.01)。结果提示，重复5次+1 OGz/455暴露复合 90dB(A)/3 omin噪声应激可引起大鼠持续性学习记忆功能障碍。 3.低G锻炼对高玲z和噪声复合应激所致大鼠学习记忆功能障 碍具有保护作用本实验在复合应激暴露之前，预先进行连续3天 (l次/d)的+4G/3min的低G锻炼，观察再暴露于高玲z和噪声复合 应激时大鼠学习记忆功能的变化。结果发现，复合应激组大鼠反应 时在暴露后各时间点较对照组均显著延长(P0 .01)，正确率均显 著下降(P0 .01);锻炼组大鼠反应时仅在暴露后即刻较对照组显著 延长(P0 .05)，而与复合应激组比较，锻炼组大鼠反应时在暴露后 各时间点均显著缩短(P0 .01)，正确率均显著升高(P0 .01)。复合 应激组大鼠总时间和错误数较对照组显著增加(P0 .01)，潜伏期显 著缩短(P0 .01);锻炼组大鼠总时间和错误数较复合应激组显著减 少(P0.01)，潜伏期显著延长(P0 .01)。结果提示，连续3天的 +4G/3min(l次/d)锻炼对+10Gz/3min复合godB(A)/30min噪声应 激所致的大鼠学习记忆功能障碍具有一定的保护作用。 总之，本研究利用动物离心机和白噪声发生器，观察了正加速 度和噪声复合应激后大鼠学习记忆能力的变化情况及其与复合应 激强度的关系，主要阐明了以下问题:高+Gz和噪声复合应激可 引起大鼠持续性学习记忆功能障碍，短时重复高+Gz暴露复合噪 声应激亦可引起大鼠持续性学习记忆功能障碍，低G锻炼对正加速 度和噪声复合应激所致的大鼠学习记忆功能障碍具有一定的保护 作用。本研究为进一步做好飞行卫生保障工作，制定安全有效的防 护措施提供了实验依据和理论指导。
[Abstract]:Because of its particularity, the modern war has a certain damage to the mental and psychological state of the part of the war. Stress stimulation is in many forms, fear, pain, hypoxia, high temperature, cold, acceleration, noise, etc. are stress sources. In the field of military medical science, the adverse effects of stress on the troops of the army are becoming more and more prominent, and the great attention of the military medical workers is gradually brought to the attention of the military medical workers. As a result of the special working environment of military flights, fighter pilots are inevitably exposed to various stress sources, among which the most common is positive acceleration (+ Gz), noise, vibration and high temperature, and has become a threat. The important factor of line security. The modern high-performance fighter has a high mobility that can be as high as + gGz in flight, up to 15-45 s, and can be repeated, and it's out of the way. Normal tolerance limits. Learning and memory are an essential brain function that is essential to the survival of animals and humans, which can reflect the gods. The functional status of the system. Previous research has confirmed that high G-value persistent positive acceleration exposure can cause brain damage and learning and memory dysfunction in animals, and a certain intensity of noise can cause human and animal The decrease of learning and memory ability. However, the effects of positive and noise composite stress factors on the brain and the protection measures It has not been reported in the study. Therefore, the change law of the working ability of the flying personnel after the effect of the above-mentioned compound stress factors is set out, and the work tool for further carrying out the flight health protection It's important to present The real meaning. The study Master's degree in the Fourth Military Medical University The effect of positive acceleration and noise compound stress on learning and memory of rats functional The purpose of this study is to reveal the positive acceleration and the noise composite stress. The change of learning and memory function in rats Law, go hand in hand to explore whether the low-G exercise is right plus Learning and memory caused by complex stress of speed and noise The animal experiment center of our school is selected in this study. The male SD rats were supplied with a z-storm on the animal from the heart machine. Lulu, at the same time using a white noise generator Noise. Y- The experiment of the labyrinth experiment, the dark-avoiding experiment and the open-field analysis experiment, respectively, in the compound stress storm Immediately, 2d, 4d and 6d after exposure, the different positive acceleration and the noise composite should be observed. under the excitation condition The change of learning and memory function in rats. The main findings and findings of this study are as follows: 1. High + Gz and noise compound stress can cause persistent learning and memory impairment in rats This experiment was in the way of observation + 10Gz/3min鍜実odB(A) The effect of/3-in-noise compound stress on learning and memory function in rats. The results showed that the correct rate of the rats in the compound stress group was at the same time. The time points are better after exposure The group and the noise group were significantly reduced (P0.01), and the reaction time Significant extension (P0.01) ; the dwell time of the central cell was compared to the control group and the noise immediately after exposure The acoustic group was significantly prolonged (P0.01); the total time and number of errors were immediately after exposure A significant increase in the irradiation group (P0 .01) The incubation period was significantly shortened immediately after exposure (P0.01). Compared with the control group at 6 d after exposure, the control group was significantly shortened (P0.01). ). Compared with the + l OGz group, the rats in the compound stress group were The rate of certainty is exposed The reaction time was significantly lower (P0.05), and the reaction time was significant. Extension (P0.01). As a result, + 10 Gz/3 min and 90 dB (A) /30min The effect of noise compound stress on persistent learning in rats memristor dysfunction, the two factors have a synergistic effect, in which + Gz It is the main influencing factor. 2. Short-time repetition of high + Gz exposure complex The effect of combined noise stress on the persistence of rats study Remembering dysfunction This experiment observed a repeat of 5 + l 002/4 55 Exposure to composite 90 dB (A)/30 min noise stress large The effect of the learning and memory function of the rat. The result shows that the compound should The correct rate of the rats in the stimulated group was significantly lower than that of the control group after exposure (P 0.01), and the reaction time is obviously prolonged. (P0.01): Total time and error The number is after the exposure, that is, There was a significant increase (P0.01). The correct rate of rats in the compound stress group was higher than that of the + Gz repeated exposure group. After exposure, all time points were significantly reduced (P0.01), and at the time of the reaction, the time points were exposed. Master's degree in the Fourth Military Medical University The total time and number of errors immediately after exposure were significantly prolonged (P0.01). Increase (P0.01). The result prompt, repeat 5 times + 1 OGz /455 Exposure to composite 90dB (A)/3-in noise Stress can cause persistent learning and memory dysfunction in rats. 3. Low G exercise The effect of high-Ling z and noise compound stress on the learning and memory function of the rats Prior to the exposure, a continuous 3-day continuous ( 1/ d) + 4G/3 min of low G exercise, observed to be exposed to high The function of learning and memory of the rats at the time of the compound stress of Ling z and the noise The results showed that the response of the compound stress group to the rats at the time of the storm The time point after exposure was significantly prolonged in the control group (P0.01), and the correct rate was significant. to descend (P0.01); exercise in group rat, only at that time of the reaction, Immediately after exposure, the control group was significantly prolonged (P0.05). ), and In comparison with the compound stress group, the exercise group rats were exposed after exposure Significant shortening of all time points (P0
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2004
【分类号】：R82
本文编号：2447476
[Abstract]:Because of its particularity, the modern war has a certain damage to the mental and psychological state of the part of the war. Stress stimulation is in many forms, fear, pain, hypoxia, high temperature, cold, acceleration, noise, etc. are stress sources. In the field of military medical science, the adverse effects of stress on the troops of the army are becoming more and more prominent, and the great attention of the military medical workers is gradually brought to the attention of the military medical workers. As a result of the special working environment of military flights, fighter pilots are inevitably exposed to various stress sources, among which the most common is positive acceleration (+ Gz), noise, vibration and high temperature, and has become a threat. The important factor of line security. The modern high-performance fighter has a high mobility that can be as high as + gGz in flight, up to 15-45 s, and can be repeated, and it's out of the way. Normal tolerance limits. Learning and memory are an essential brain function that is essential to the survival of animals and humans, which can reflect the gods. The functional status of the system. Previous research has confirmed that high G-value persistent positive acceleration exposure can cause brain damage and learning and memory dysfunction in animals, and a certain intensity of noise can cause human and animal The decrease of learning and memory ability. However, the effects of positive and noise composite stress factors on the brain and the protection measures It has not been reported in the study. Therefore, the change law of the working ability of the flying personnel after the effect of the above-mentioned compound stress factors is set out, and the work tool for further carrying out the flight health protection It's important to present The real meaning. The study Master's degree in the Fourth Military Medical University The effect of positive acceleration and noise compound stress on learning and memory of rats functional The purpose of this study is to reveal the positive acceleration and the noise composite stress. The change of learning and memory function in rats Law, go hand in hand to explore whether the low-G exercise is right plus Learning and memory caused by complex stress of speed and noise The animal experiment center of our school is selected in this study. The male SD rats were supplied with a z-storm on the animal from the heart machine. Lulu, at the same time using a white noise generator Noise. Y- The experiment of the labyrinth experiment, the dark-avoiding experiment and the open-field analysis experiment, respectively, in the compound stress storm Immediately, 2d, 4d and 6d after exposure, the different positive acceleration and the noise composite should be observed. under the excitation condition The change of learning and memory function in rats. The main findings and findings of this study are as follows: 1. High + Gz and noise compound stress can cause persistent learning and memory impairment in rats This experiment was in the way of observation + 10Gz/3min鍜実odB(A) The effect of/3-in-noise compound stress on learning and memory function in rats. The results showed that the correct rate of the rats in the compound stress group was at the same time. The time points are better after exposure The group and the noise group were significantly reduced (P0.01), and the reaction time Significant extension (P0.01) ; the dwell time of the central cell was compared to the control group and the noise immediately after exposure The acoustic group was significantly prolonged (P0.01); the total time and number of errors were immediately after exposure A significant increase in the irradiation group (P0 .01) The incubation period was significantly shortened immediately after exposure (P0.01). Compared with the control group at 6 d after exposure, the control group was significantly shortened (P0.01). ). Compared with the + l OGz group, the rats in the compound stress group were The rate of certainty is exposed The reaction time was significantly lower (P0.05), and the reaction time was significant. Extension (P0.01). As a result, + 10 Gz/3 min and 90 dB (A) /30min The effect of noise compound stress on persistent learning in rats memristor dysfunction, the two factors have a synergistic effect, in which + Gz It is the main influencing factor. 2. Short-time repetition of high + Gz exposure complex The effect of combined noise stress on the persistence of rats study Remembering dysfunction This experiment observed a repeat of 5 + l 002/4 55 Exposure to composite 90 dB (A)/30 min noise stress large The effect of the learning and memory function of the rat. The result shows that the compound should The correct rate of the rats in the stimulated group was significantly lower than that of the control group after exposure (P 0.01), and the reaction time is obviously prolonged. (P0.01): Total time and error The number is after the exposure, that is, There was a significant increase (P0.01). The correct rate of rats in the compound stress group was higher than that of the + Gz repeated exposure group. After exposure, all time points were significantly reduced (P0.01), and at the time of the reaction, the time points were exposed. Master's degree in the Fourth Military Medical University The total time and number of errors immediately after exposure were significantly prolonged (P0.01). Increase (P0.01). The result prompt, repeat 5 times + 1 OGz /455 Exposure to composite 90dB (A)/3-in noise Stress can cause persistent learning and memory dysfunction in rats. 3. Low G exercise The effect of high-Ling z and noise compound stress on the learning and memory function of the rats Prior to the exposure, a continuous 3-day continuous ( 1/ d) + 4G/3 min of low G exercise, observed to be exposed to high The function of learning and memory of the rats at the time of the compound stress of Ling z and the noise The results showed that the response of the compound stress group to the rats at the time of the storm The time point after exposure was significantly prolonged in the control group (P0.01), and the correct rate was significant. to descend (P0.01); exercise in group rat, only at that time of the reaction, Immediately after exposure, the control group was significantly prolonged (P0.05). ), and In comparison with the compound stress group, the exercise group rats were exposed after exposure Significant shortening of all time points (P0
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2004
【分类号】：R82
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