模拟失重30 d后再超重对猴牙龈组织趋化因子CCL20及其受体CCR6表达的影响

发布时间：2019-05-11 09:13

【摘要】：目的:探讨模拟失重30 d后再超重对猴牙龈组织趋化因子CCL20及其受体CCR6表达的影响。方法:23只雄性猕猴随机分为4组,即对照组(A)、失重组(B)、超重组(C)、模拟失重30 d后再超重组(D)。其中,D组根据载荷量大小又分为4个亚组,即+11Gx/270 s组(D1)、+13Gx/230 s组(D2)、+15 Gx/200 s组(D3)和+13 Gx/230 s恢复9 d组(D4)。采用组织学方法观察牙龈的形态学变化,通过免疫组织化学、Q-PCR方法检测牙龈组织中CCL20、CCR6的表达情况。采用SPSS17.0软件包对数据进行统计学分析。结果:各实验组中牙龈结构均未见明显损伤,但实验组中的淋巴细胞及中性粒细胞浸润较对照组多。对照组牙龈组织几乎不表达CCL20,但弱表达CCR6;实验组CCL20阳性表达主要位于牙龈上皮组织的基底层,CCR6阳性表达主要位于牙龈上皮棘层和基底层。除失重组的CCL20外,各实验组牙龈组织中的CCL20和CCR6在蛋白水平、mRNA水平上的表达均显著高于对照组(P0.05)。结论:模拟失重30 d后再超重环境中,猴牙龈组织结构未见明显改变,但牙龈组织趋化因子CCL20及其受体CCR6表达明显增强。
[Abstract]:Aim: to investigate the effect of overweight on the expression of chemokine CCL20 and its receptor CCR6 in gum tissue of monkeys after 30 days of simulated weightlessness. Methods: 23 male rhesus monkeys were randomly divided into 4 groups: control group (A), lost recombinant (B), superrecombinant (C), simulated weightlessness for 30 days, and then superrecombinant (D). Among them, group D was divided into four subgroups according to the load size, namely, 11Gx/270 s group (D1), 13Gx/230 s group (D2), 15 Gx/200 s group (D 3) and 13Gx/230 s recovery 9 d group (D4). The morphological changes of gums were observed by histology, and the expression of CCL20,CCR6 in gum tissues was detected by immunohistochemistry and Q-PCR. SPSS17.0 software package was used to analyze the data statistically. Results: there was no obvious damage to gum structure in each experimental group, but the infiltration of lymphocytes and neutrophils in the experimental group was more than that in the control group. In the control group, there was almost no expression of CCL20, in gum tissue, but the positive expression of CCL20 in the experimental group with weak expression of CCR6; was mainly located in the basal layer of the gum epithelial tissue, and the positive expression of CCR6 was mainly located in the spinous layer and basal layer of the gum epithelial tissue. Except for the lost CCL20, the expression of CCL20 and CCR6 in the gum tissue of each experimental group was significantly higher than that of the control group at the protein level and mRNA level (P 0.05). Conclusion: in the overweight environment after 30 days of simulated weightlessness, the gum tissue structure of monkeys did not change significantly, but the expression of chemokine CCL20 and its receptor CCR6 in gum tissue was significantly increased.
【作者单位】：解放军医学院;解放军第306医院全军口腔疾病诊治中心;北京特种医学实验中心;
【基金】：国家自然科学基金(30870598) 全军医药卫生科研基金(06MA004)~~
【分类号】：R856.78

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