海水淹溺型急性肺损伤兔外周血中性粒细胞凋亡的动态变化
发布时间：2019-05-20 20:27
【摘要】：目的探讨海水淹溺型急性肺损伤(SWD-ALI)时外周血中性粒细胞(PMN)凋亡的演变规律以及细胞因子对其可能的影响。方法36只新西兰兔用随机数字表法均分为对照组(作为0h基线值)和海水灌注后1、3、6、12、24h组(分别于灌注后1、3、6、12、24h处死)。观察各组血象和血气分析指标的变化,计算肺湿/干重比(W/D)和肺微血管通透指数;流式细胞术检测PMN凋亡情况,ELISA法检测血清TNF-α、IL-1β、IL-10水平;对肺组织进行病理学观察,并计算病理评分(LPS)。结果W/D值在3h达高峰,肺微血管通透指数以6h最高,炎症细胞浸润以6～12h最显著。LPS在1h已显著高于对照组(P0.01),6h达最高。PMN凋亡率于灌注后1h一过性升高,3h显著抑制,6h最低,以后逐渐接近对照水平。PMN计数与凋亡率呈负相关(P0.05)。各海水灌注组血清TNF-α、IL-1β、IL-10均显著高于对照组(P0.05或P0.01),与LPS呈正相关(P0.05或P0.01),但与PMN凋亡率无相关性(P0.05)。结论PMN凋亡在SWD-ALI炎症反应早期受到显著抑制,是最主要的炎症效应细胞。TNF-α、IL-1β、IL-10在SWD-ALI中发挥重要作用,但对PMN凋亡抑制无显著影响。
[Abstract]:Objective to investigate the evolution of (PMN) apoptosis in peripheral blood neutrophils during seawater drowning acute lung injury (SWD-ALI) and the possible effect of cytokines on it. Methods 36 New Zealand rabbits were randomly divided into control group (as 0 h baseline value) and 1, 3, 6, 12, 24 h group (1, 3, 6, 12, 24 h after seawater perfusion, respectively). The changes of hemogram and blood gas analysis indexes were observed, the lung wet / dry weight ratio (W 鈮，
本文编号：2481934
[Abstract]:Objective to investigate the evolution of (PMN) apoptosis in peripheral blood neutrophils during seawater drowning acute lung injury (SWD-ALI) and the possible effect of cytokines on it. Methods 36 New Zealand rabbits were randomly divided into control group (as 0 h baseline value) and 1, 3, 6, 12, 24 h group (1, 3, 6, 12, 24 h after seawater perfusion, respectively). The changes of hemogram and blood gas analysis indexes were observed, the lung wet / dry weight ratio (W 鈮，
本文编号：2481934
本文链接：https://www.wllwen.com/yixuelunwen/yxlw/2481934.html
最近更新
教材专著
