模拟战伤应激性脑缺血大鼠尼莫地平、音乐协同高压氧治疗观察

发布时间：2019-07-11 15:46

【摘要】：目的通过观察尼莫地平、音乐协同高压氧(HBO)综合治疗电击、创伤产生的应激性缺血性脑血管病,进一步探讨HBO、尼莫地平、音乐在缺血性脑血管病(ICVD)治疗中的作用。方法用电击、创伤模拟战时伤亡刺激大鼠视、听觉,产生应激,再结扎双侧颈总动脉(A),阻断血流20min后恢复血流灌注,给予HBO+音乐(应激1组)、HBO+尼莫地平+音乐(应激2组)和HBO(应激3组)治疗,检测血小板-CD62p(platelet-selection-CD62p,CD62p),红细胞内钙(intraerythrocytic-calcium,IECa2+),红细胞变形指数(dyformity-index,DI),皮质醇(cortisol,Cor)。断头取脑作形态学观察,并与对照组对比。结果应激1、2、3组大鼠CD62p、IECa2+、Cor含量明显高于对照组(P0.05),以应激3组更显著(P0.01),应激1组次之。DI值应激1、3组明显低于对照组和应激2组(P0.05),以应激3组更显著(P0.01)。脑组织形态学应激1、3组神经细胞数量减少,残存的细胞大部分肿胀,固缩、变性并崩解。应激2组仅见少数散在神经细胞肿胀、固缩、变性。结论尼莫地平、音乐配合HBO治疗有益于应激性ICVD的康复。
[Abstract]:Objective to observe the effect of nimodipine, music combined with hyperbaric oxygen (HBO) on stress ischemic cerebrovascular disease caused by shock and trauma, and to further explore the role of HBO, nimodipine and music in the treatment of ischemic cerebrovascular disease (ICVD). Methods the rats were stimulated by electric shock and trauma to stimulate vision, hearing and stress, and then ligated bilateral common carotid artery (A), to block blood flow 20min and then resume blood perfusion. HBO music (stress group 1), HBO nimodipine music (stress group 2) and HBO (stress group 3) were used to detect platelet-CD62p (platelet-selection-CD62p,CD62p) and erythrocyte calcium (intraerythrocytic-calcium,IECa2). Erythrocyte deformability index (dyformity-index,DI), cortisol (cortisol,Cor). The brain was decapitated for morphological observation and compared with the control group. Results the contents of CD62p,IECa2 and Cor in group 3 were significantly higher than those in control group (P 0.05), especially in stress group 3 (P 0.01), followed by stress group 1. Di value in group 3 was significantly lower than that in control group and stress group 2 (P 0.05), especially in stress group 3 (P 0.01). The number of nerve cells in the three groups decreased, and most of the remaining cells were swollen, pyknotic, denatured and disintegrated. In the stress group 2, only a few scattered nerve cells were swollen, pyknosis and degeneration. Conclusion Nimodipine, music combined with HBO is beneficial to the rehabilitation of stress ICVD.
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【分类号】：R82

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