针刺对脑挫伤大鼠脑组织坏死面积及脑细胞凋亡的影响

发布时间：2018-02-13 15:17

本文关键词： 脑挫伤针灸疗法坏死面积细胞凋亡大鼠　出处：《中医杂志》2010年06期 　论文类型：期刊论文

【摘要】：目的观察针刺对脑挫伤模型大鼠脑组织坏死面积与脑细胞凋亡的影响。方法参照Feeney自由落体撞击造模法建立脑挫伤大鼠模型,将40只大鼠随机分为假手术组、模型组、纳络酮组、针刺组,每组10只。针刺组、纳络酮组分别给予针刺、腹腔注射纳络酮,每天1次,共7天。处理结束后,采用生物医学图像分析系统计算坏死面积、TUNEL法检测脑细胞凋亡。结果模型组大鼠脑组织损伤严重,坏死面积大于损伤面积,出现大量的脑细胞凋亡(P0.01);针刺组、纳络酮组脑组织坏死面积较模型组明显减小,且均小于损伤面积(P0.01);两组大鼠脑细胞凋亡指数较模型组明显减少(P0.01);针刺组脑组织坏死面积与纳络酮组比较无统计学意义(P0.05)。结论针刺可缩小脑挫伤模型大鼠脑织坏死面积、减少脑细胞凋亡,具有确切的抗继发性脑损伤作用。
[Abstract]:Objective to observe the effect of acupuncture on the necrotic area of brain tissue and the apoptosis of brain cells in rats with cerebral contusion. Methods according to the method of Feeney free falling body impact, 40 rats were randomly divided into sham-operation group and model group. Naloxone group (n = 10), acupuncture group (n = 10), naloxone group (n = 10), naloxone group (n = 10) and naloxone group (n = 10) were treated with naloxone once a day for 7 days. The necrosis area was calculated by biomedical image analysis system and Tunel method was used to detect the apoptosis of brain cells. Results in the model group, the brain tissue was damaged seriously, the necrotic area was larger than the damaged area, and a large number of apoptotic brain cells appeared in the model group (P 0.01). The necrotic area of brain tissue in naloxone group was significantly smaller than that in the model group. The apoptotic index of brain cells in the two groups was significantly lower than that in the model group, and the area of brain tissue necrosis in the acupuncture group was not significantly different from that in the naloxone group. Conclusion Acupuncture can reduce the area of brain tissue necrosis in the brain contusion model rats. It can reduce the apoptosis of brain cells and has the effect of anti-secondary brain injury.
【作者单位】：暨南大学医学院中医学系;广州中医药大学针灸推拿学院;
【基金】：广东省自然科学基金资助项目(06300588),广东省自然科学基金面上资助项目(9151063201000028) 暨南大学引进优秀人才科研启动基金资助项目(51207014)
【分类号】：R245

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