针刺对躁狂大鼠模型行为学影响及作用机制的研究

发布时间：2018-02-21 00:58

本文关键词： 躁狂症丙戊酸钠针刺实验研究　出处：《广州中医药大学》2013年博士论文　论文类型：学位论文

【摘要】：研究背景我们以针刺对躁狂大鼠模型对行为学和部分中枢神经递质水平的影响为研究重点,从宏观和分子生物学探索针灸治病起效机制,为明确针灸治疗躁狂症提供实验依据。研究目的通过观察针刺经穴、非穴以及丙戊酸钠对跺狂大鼠模型体重、进食量和睡眠潜伏期的影响,并通过检测大鼠海马脑区GABA. Glu含量及EAAT3蛋白表达、外周血神经递质(多巴胺、5-HT)含量变化,初步探讨针刺治疗躁狂症的疗效及作用机制。研究方法 SD大鼠,SPF级,雌雄各半,体重160-200g,共50只,饲养1周后随机分为空白对照组、模型组、丙戊酸钠组、穴位针刺组和非穴位针刺组,每组10只。采用改良多平台睡眠剥夺法(MMPM)建立躁狂模型。空白组和模型组不做任何干预,穴位针刺穴位组及非穴组大鼠给予针刺干预,每次留针30分钟,每日1次,丙戊酸钠组,予丙戊酸钠300mg/kg体重,溶于2m1纯净水中灌胃,1次/天。水环境处理7天结束后观察各组大鼠的精神、行为、记录脱离水环境到第一次进入睡眠的时间(即睡眠潜伏期)和12小时进食量,并进行旷场试验。第8天处死所有大鼠,检测大鼠海马脑区GABA及G1u及基因表达、外周血多巴胺类神经递质含量。所有数据使用SPSS13.0进行统计分析。研究结果 1.针刺穴位组、丙戊酸钠组与模型组比较,体重、12h进食量及情绪兴奋差异均有统计学意义(P0.01),提示丙戊酸钠和穴位针刺能缓解躁狂症对体重、12h进食量及情绪兴奋的影响,非穴位组跟模型组比较,差异无统计学意义(P0.05),提示非穴位对体重、12h进食量及情绪兴奋无改善作用。丙戊酸钠组和穴位组比较,差异无统计学意义(P0.05),对于针刺及丙戊酸钠两者效果差别不大。丙戊酸钠组、穴位组与非穴位组跟模型组比较,对睡眠潜伏期的改善差异有极显著统计学意义,提示三种方法均能明显改善躁狂症导致的睡眠潜伏期延长。丙戊酸钠组和穴位组比较,差异有统计学意义(P0.01),对于缓解躁狂症导致睡眠潜伏期延长,穴位针刺效果更佳。 2.在调节海马脑区GLU/GABA含量作用中,丙戊酸钠组、穴位组跟模型组比较,差异有统计学意义(P0.01)。 3.躁狂大鼠模型外周血DA及5-TH水平比较,丙戊酸钠组、穴位组跟模型组比较,差异有统计学意义(P0.01)。 4.针刺穴位治疗后EAAT3蛋白表达增高,可以看到明显的蛋白电泳条带,丙戊酸钠对照也比较明显,而非穴组则不明显。研究结论 1.丙戊酸钠组、会刺穴位组能有效增加躁狂大鼠体重及12h进食量、缩短睡眠潜伏期,缓解情绪兴奋,针刺穴位组的治疗效应略优于丙戊酸钠组,针刺非穴位无明显治疗效应。 2.丙戊酸钠组及针刺穴位组能有效降低海马脑区Glu、提高GABA含量,针刺非穴位无明显治疗效应。 3.丙戊酸钠组及针刺穴位组能有效降低躁狂大鼠模型外周血DA水平,增高5-TH水平。 4.针刺穴位制狂效应可能与增加海马脑区EAAT3蛋白表达有关。
[Abstract]:Research background. We focus on the effect of acupuncture on behavior and some central neurotransmitters in manic rats. We explore the mechanism of acupuncture and moxibustion in treating mania from macroscopical and molecular biology, and provide experimental basis for the treatment of mania by acupuncture and moxibustion. Research purpose. The effects of acupuncture at acupoints, non-acupoints and sodium valproate on body weight, food intake and sleep latency in rats were observed. The contents of GABA 路Glu and the expression of EAAT3 protein in hippocampus of rats were measured. The changes of peripheral blood neurotransmitters (dopamine 5-HT) were studied in order to explore the therapeutic effect and mechanism of acupuncture on mania. Research method. SD rats with SPF grade, male and female, weight 160-200g, were randomly divided into blank control group, model group, sodium valproate group, acupoint acupuncture group and non-acupoint acupuncture group. There were 10 rats in each group. The manic model was established by modified multi-platform sleep deprivation (MMPM). The blank group and the model group did not do any intervention. The rats of acupoint acupuncture group and non-point group were treated with acupuncture intervention for 30 minutes each time, once a day. Sodium valproate group was given sodium valproate 300 mg / kg body weight, dissolved in 2m-1 purified water once a day. After 7 days of water treatment, the mental and behavior of rats in each group were observed. The time (i.e. sleep latency) and the amount of food consumed for 12 hours from the water environment to the first sleep were recorded, and the open field test was carried out. On the 8th day, all the rats were killed and the GABA, G1u and gene expression in the hippocampal brain of the rats were detected. The contents of dopamine neurotransmitters in peripheral blood were analyzed by SPSS13.0. Research results. 1.Compared with the model group, there were significant differences in food intake and emotional excitement between acupuncture acupoint group and valproate sodium group, suggesting that sodium valproate and acupuncture at acupoint could alleviate the effects of mania on body weight, food intake and emotional excitement in 12 hours. There was no significant difference between the non point group and the model group (P 0. 05), which suggested that the non acupoint had no effect on the body weight intake and emotional stimulation for 12 hours, while the sodium valproate group and the acupoint group had no effect on the improvement. There was no significant difference in the effect of acupuncture and sodium valproate between the two groups. There was significant difference in the improvement of sleep latency between the point group, the point group and the non-point group as compared with the model group, and there was significant difference in the improvement of sleep latency between the two groups. The results indicated that all the three methods could obviously improve the prolonged sleep latency caused by mania, and the difference between valproate sodium group and acupoint group was statistically significant (P 0.01), which was better for relieving mania and prolonging sleep latency. The effect of acupuncture at acupoint was better than that of sodium valproate group. 2. In regulating the content of GLU/GABA in hippocampus, there was a significant difference between the sodium valproate group and the acupoint group as compared with the model group (P 0.01). 3.The levels of DA and 5-TH in peripheral blood of manic rats were compared with those of model group, and the difference was statistically significant in sodium valproate group, acupoint group and model group. 4.After acupuncture, the expression of EAAT3 protein increased, and the protein electrophoresis bands were observed. The control group of valproate sodium valproate was also obvious, but the non-point group was not. Research conclusion. 1.Sodium valproate group, acupoint acupuncture group can effectively increase the body weight and 12h food intake of manic rats, shorten sleep latency and relieve emotional excitement. The therapeutic effect of acupuncture acupoint group is slightly better than that of sodium valproate group, but acupuncture has no obvious therapeutic effect at non-acupoints. 2. Sodium valproate and acupoint acupuncture group can effectively reduce Glu-induced hippocampal area and increase the content of GABA. Acupuncture at non-acupoint has no obvious therapeutic effect. 3. Sodium valproate and acupoint acupuncture group could effectively decrease DA level and increase 5-TH level in peripheral blood of manic rats. 4. The effect of acupuncture on derangement may be related to the increase of EAAT3 protein expression in hippocampus.
【学位授予单位】：广州中医药大学
【学位级别】：博士
【学位授予年份】：2013
【分类号】：R246.6;R-332

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