穴位注射组胺在急性佐剂性关节炎大鼠上引起的镇痛效应

发布时间：2018-03-04 00:24

本文选题：急性关节炎　切入点：针刺　出处：《针刺研究》2010年02期 　论文类型：期刊论文

【摘要】：目的:观察组胺对穴位处肥大细胞的激活作用,以及其引起的类似针刺的镇痛效应,讨论穴位注射疗法可能存在的机制。方法:SD大鼠60只,随机分为空白对照组、模型组、生理盐水对照组、手针组和组胺注射组。大鼠左踝关节腔注射完全弗氏佐剂0.05 mL制作佐剂性关节炎模型。测量各组痛阈变化,并对穴位组织进行组织学观察,统计肥大细胞脱颗粒率。比较在"足三里"穴位注射100μg/mL的组胺溶液50μL和手针刺激20 min下的镇痛效应的差异性。结果:组胺注射和手针针刺,都能提高大鼠的痛阈达到镇痛效果,与模型组相比治疗后痛阈都有显著提高(P0.01)。组胺引起的肥大细胞脱颗粒率与生理盐水相比明显增高(P0.01),与针刺相比差异无统计学意义(P0.05)。结论:在佐剂性关节炎大鼠模型中,组胺可以引起穴位处肥大细胞脱颗粒,产生与针刺类似的镇痛效应。以刺激肥大细胞为目的的新型穴位注射疗法存在可行性。
[Abstract]:Objective: to observe the effect of histamine on the activation of mast cells at acupoints and the analgesic effect induced by acupuncture, and to discuss the possible mechanism of acupoint injection therapy. Methods: sixty Sprague-Dawley rats were randomly divided into blank control group and model group. Normal saline control group, hand acupuncture group and histamine injection group. The model of adjuvant arthritis was made by injecting the complete Freund's adjuvant 0.05 mL into the left ankle joint of rats. The pain threshold was measured in each group, and the tissue of acupoint was observed histologically. The difference of analgesic effect between 100 渭 g / mL histamine solution injected at Zusanli acupoint and 20 min after hand acupuncture was compared. Results: histamine injection and hand acupuncture acupuncture could improve the pain threshold of rats to achieve analgesic effect. Compared with the model group, the pain threshold increased significantly after treatment. The degranulation rate of mast cells induced by histamine was significantly higher than that of normal saline, but there was no significant difference compared with acupuncture. Conclusion: in the model of adjuvant arthritis, the degranulation rate of mast cells is significantly higher than that of normal saline. Histamine can induce degranulation of mast cells at acupoints and produce analgesic effects similar to acupuncture. A new type of acupoint injection therapy aimed at stimulating mast cells is feasible.
【作者单位】：复旦大学力学与工程科学系;上海市针灸经络研究中心;上海中医药大学;
【基金】：上海市重点学科建设资助项目(No.B112、S30340、T0302) 上海市科技发展基金(No.06DZ19732、064319053、07DZ19722) 上海市科委科研平台建设专项(No.2008DZ1973503) 国家重点基础研究发展计划(“973”项目,No.2005CB523306)
【分类号】：R245

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