针刺对2型糖尿病大鼠胰岛β细胞形态学影响

发布时间：2018-03-12 15:43

本文选题：针刺　切入点：型糖尿病　出处：《中华中医药杂志》2010年12期 　论文类型：期刊论文

【摘要】：目的:研究针刺对2型糖尿病大鼠胰岛β细胞形态结构的作用。方法:将造模成功的大鼠按随机化原则分为针刺组、西药组、安慰剂组,同时将普食组大鼠作为正常对照组,每组10只。针刺组大鼠取"后三里"(相当于足三里)、内庭和胰俞穴给与针刺治疗,西药组用罗格列酮(0.2mg/kg体质量)灌胃,安慰剂组用双蒸水(2mL/kg体质量)灌胃,1次/d,连续4周。观察治疗前后空腹血糖、血脂变化,实验结束时,分离胰腺,石蜡包埋、酒精脱水及胰岛素(Ins)免疫组化染色后,光镜观察胰岛β细胞形态及胰岛细胞胰岛素表达。结果:针刺组胰腺腺泡、胰岛结构完整,胰岛内细胞排列大体规整,大小、形态接近正常,胞浆肿胀、纤维组织增生少见,改变显著优于西药组而接近正常组;胰岛细胞胰岛素表达针刺组显著高于安慰剂组。结论:针刺具有保护胰岛β细胞形态的作用。
[Abstract]:Objective: to study the effect of acupuncture on the morphology and structure of islet 尾 cells in type 2 diabetic rats. Methods: the successful rats were randomly divided into acupuncture group, western medicine group, placebo group and normal control group. There were 10 rats in each group. The rats in the acupuncture group were treated with acupuncture at Zusanli (equivalent to Zusanli Li, et al and Yanchu), and those in the western medicine group were treated with rosiglitazone 0.2 mg / kg body mass by stomach, and the rats in the western medicine group were perfused with rosiglitazone 0.2 mg / kg body mass. The placebo group was fed with double steamed water at 2ml / kg body mass once a day for 4 weeks. The changes of fasting blood glucose and blood lipid were observed before and after treatment. At the end of the experiment, the pancreas was separated, paraffin embedded, alcohol dehydration and insulin Ins were stained by immunohistochemistry. Results: in the acupuncture group, the pancreatic acinar structure was intact, the islet cells were arranged regularly, the size and shape of the islets were close to normal, the cytoplasm was swollen, and the proliferation of fibrous tissue was rare. The change of insulin expression in islet cells in acupuncture group was significantly higher than that in placebo group.
【作者单位】：南京中医药大学针药结合实验室;山东省济宁医学院;
【基金】：国家自然科学基金资助项目(No.30772827)~~
【分类号】：R245

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