电针干预骨癌痛大鼠的实验研究

发布时间：2018-06-07 14:41

本文选题：电针 + 骨癌痛　；参考：《黑龙江中医药大学》2013年博士论文

【摘要】：目的：通过电针刺激乳腺癌骨转移大鼠,观察电针对骨癌痛的影响,讨论其可行性,并探讨电针治疗骨癌痛的作用机理,以指导临床运用方法：1、将24只体重180-220g的SD雌性大鼠,随机分为三组：空白组、假手术组、模型组。将Walker256大鼠乳腺癌细胞经腹水传代后(1×105cells)接种至SD大鼠左侧后肢胫骨骨髓腔内制备模型。与空白组、假手术组比较判断造模成败。2、将40只体重180-220g的SD雌性大鼠接种Walker256大鼠乳腺癌细胞造模后随机分为5组：空白组、模型组、电针组、药物组、针药组,每组8只,分别干预后,观察各组大鼠行为学、形态学及利用免疫组化法和Western-blot法观察抗酒石酸酸性磷酸酶(Tartrate resistant acid phosphatase,TRAP)、胶质纤维酸性蛋白(Glial Fibrillary Acidic Protein,GFAP)的表达。结果： ].大鼠接种Walker256乳腺癌细胞后,X线摄片可见术侧胫骨在接种后8天、14天和21天分别出现不同程度的骨质损伤和破坏双侧后肢热痛过敏,鼠尾出现机械性触痛过敏；术侧胫骨HE染色在接种后21天可见大量的肿瘤细胞； 2.大鼠接种后21天胫骨重酒石酸酸性磷酸酶(TRAP)染色表达上调,同时激活脊髓胶质细胞的标志物GFAP; 3.应用电针、唑来膦酸及电针+唑来膦酸治疗均能够使上调的TRAP表达下降,同时抑制被激活的GFAP; 4.电针+唑来膦酸对骨癌痛的治疗作用明显优于单纯电针或唑来膦酸的作用。结论： 1.利用Walker256乳腺癌细胞能够成功制出大鼠乳腺癌骨转移模型 2.应用电针、药物及针药结合均可缓解大鼠骨癌痛,且针药结合疗效明显优于单纯电针或药物治疗； 3.电针对癌症骨转移疼痛具有治疗作用,而且电针结合药物能够提高镇痛效果。
[Abstract]:Objective: to observe the effect of electroacupuncture on bone cancer pain, discuss its feasibility and explore the mechanism of electroacupuncture in treating bone cancer pain in rats with bone metastases of breast cancer. Methods Twenty four female SD rats weighing 180-220 g were randomly divided into three groups: blank group, sham operation group and model group. Walker256 rat breast cancer cells were inoculated into the left hindlimb tibial medullary cavity of SD rats after ascites passage and 1 脳 105 cells were inoculated into the tibial medullary cavity of the left hind limb to make the model. 40 SD female rats weighing 180-220 g were randomly divided into 5 groups: blank group, model group, electroacupuncture group, drug group, acupuncture group, and acupuncture group. After intervention, the behavior, morphology and the expression of tartrate resistant acid phosphatase, glial fibrillary acidic protein (Glial Fibrillary Acidic protein Western-blot) were observed by immunohistochemical and Western-blot methods. Results: ]. After Walker256 breast cancer cells were inoculated, the tibia of the operative side appeared different degrees of bone injury and hyperthermia on the 14th and 21st days after inoculation, and the mechanical tenderness was found in the tail of the rat. A large number of tumor cells were observed 21 days after inoculation with HE staining in the operative tibia. 2. The expression of tibial heavy tartrate acid phosphatase (TRAP) was up-regulated 21 days after inoculation, and the marker of glial cells in spinal cord was activated. 3. Electroacupuncture, zoledronic acid and electroacupuncture zoledronic acid could decrease the up-regulated expression of TRAP and inhibit the activated GFAPs. 4. The therapeutic effect of electroacupuncture zoledronic acid on bone cancer pain is better than that of electroacupuncture alone or zoledronic acid alone. Conclusion: 1. Bone Metastasis Model of Breast Cancer in Rats can be successfully established by using Walker256 Breast Cancer cells 2. The combination of electroacupuncture, medicine and acupuncture can relieve the pain of bone cancer in rats, and the curative effect of acupuncture combined with medicine is better than that of simple electroacupuncture or drug therapy. 3. Electroacupuncture can treat cancer bone metastases pain, and electroacupuncture combined with drugs can improve analgesic effect.
【学位授予单位】：黑龙江中医药大学
【学位级别】：博士
【学位授予年份】：2013
【分类号】：R245.97;R273

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