口面部穴位对内脏感觉的调控作用及其途径

发布时间：2018-07-24 18:09

【摘要】：目的探讨电针口面部穴位对内脏感觉的调控作用及其途径。方法成年SD大鼠30只,随机分为生理盐水组、内脏痛组、四白穴组、阳白穴组、颊车穴组,每组6只。生理盐水组腹腔注射0.9%生理盐水(1ml/100g),不给予其他任何处理;内脏痛组腹腔注射乙酸造成内脏痛模型后不给予其他任何处理;四白穴组先电针双侧四白穴20min,再腹腔注射乙酸造成内脏痛模型;阳白穴组和颊车穴组除电针穴位不同外,其余处理同四白穴组。处理完毕后,观察大鼠的行为学变化(扭体反应)以及孤束核(NTS)和三叉旁核(PTN)的c-fos表达。结果 (1)大鼠腹腔注射0.6%的乙酸后,引发典型的内脏痛行为反应(扭体反应)。电针四白穴和颊车穴预处理后,由内脏痛引发的腹部收缩次数明显减少,与内脏痛组比较均有显著差异(P0.01,P0.05);电针阳白穴对内脏痛大鼠腹部收缩的次数无明显影响。(2)电针四白穴预处理后,内脏痛所致NTS的c-fos表达明显减少,与内脏痛组比较有显著差异(P0.05);电针颊车穴亦产生类似的作用;电针阳白穴作用不明显。(3)电针阳白穴预处理PTN的c-fos阳性神经元数目与内脏痛组比较无明显变化。电针四白穴预处理后,三叉旁核的c-fos表达较阳白穴组明显增加(P0.01);电针颊车穴亦出现与四白穴类似的效应。结论 (1)电针口面部穴位对大鼠内脏痛有显著的镇痛效应,且表现出一定的穴位特异性,与穴位的神经支配密切相关。(2)面口部穴位的躯体感觉传入可能经PTN中继后与胃肠道的内脏感觉传入在NTS发生汇聚并进行整合,PTN-NTS组成的二级神经元传入通路可能是面口部穴位调节内脏功能活动的重要途径。
[Abstract]:Objective to investigate the effect and pathway of electroacupuncture on visceral sensation. Methods Thirty adult SD rats were randomly divided into normal saline group, visceral pain group, Sibai point group, Yangbai point group and buccal vehicle point group with 6 rats in each group. In the saline group, 0.9% saline (1ml/100g) was injected intraperitoneally without any other treatment, and in the visceral pain group, the visceral pain model was induced by intraperitoneal injection of acetic acid without any other treatment. The model of visceral pain was induced by intraperitoneal injection of acetic acid in the Sibai acupoint group and the Yangbai acupoint group and the buccal che acupoint group in addition to the different electroacupuncture points, the rest were treated with the same Sibai point group. After treatment, the behavioral changes (writhing reaction) and the c-fos expression of (NTS) and (PTN) in the nucleus tractus solitarii were observed. Results (1) the typical visceral pain response (writhing reaction) was induced by intraperitoneal injection of 0.6% acetic acid in rats. The number of abdominal contractions induced by visceral pain was significantly decreased after electroacupuncture at Sibai point and buccal point. Compared with the visceral pain group, there was significant difference (P0.01P05), and the number of abdominal contractions in rats with visceral pain was not significantly affected by electroacupuncture at Yang Bai point. (2) the c-fos expression of NTS induced by visceral pain was significantly decreased after electroacupuncture at Sibai point preconditioning. Compared with visceral pain group, there was significant difference (P0.05); electroacupuncture at buccal car point also had similar effect; electroacupuncture at Yang Bai point had no obvious effect. (3) the number of c-fos positive neurons in PTN pretreated with electroacupuncture at Yangbai point had no significant change compared with visceral pain group. The expression of c-fos in the trigeminal nucleus was significantly increased after electroacupuncture at Sibai point (P0.01), and the effect was similar to that at Sibai point. Conclusion (1) electroacupuncture at facial points has a significant analgesic effect on visceral pain in rats, and has a certain acupoint specificity. (2) somatosensory afferent of facial and oral acupoints may pass through PTN relay and the secondary neuronal afferent pathway composed of PTN-NTS may converge with gastrointestinal visceral sensory afferent in NTS. The important way of regulating visceral function at facial and oral acupoints.
【作者单位】：广东省中医院针灸科广东省中医院针灸效应规律及机制重点研究室;
【基金】：国家自然科学基金资助项目(30200356)
【分类号】：R245

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