穴位埋线合并胰岛素对拟阿尔茨海默病大鼠学习记忆的影响

发布时间：2018-07-26 10:36

【摘要】：目的观察穴位埋线和胰岛素联合应用对拟阿尔茨海默病(Alzheimer’s disease,AD)大鼠学习记忆的影响,并探讨其可能的治疗机制。方法大鼠海马CA1区微量注射冈田酸(Okadaic acid,OA)建立拟AD模型大鼠。肾腧、大椎穴位埋线、胰岛素侧脑室注射。用Morris水迷宫实验检测其学习记忆能力,电镜观察海马CA1区神经元超微结构变化,免疫组化方法观察胆碱乙酰转移酶(ChAT)的表达。结果与模型组比较,穴位埋线组、联合组的学习记忆能力明显改善(P0.01),海马CA1区神经元凋亡现象明显改善,ChAT表达增加(P0.05),联合组更为显著(P0.05)。结论穴位埋线和胰岛素联合应用对拟AD大鼠学习记忆的改善作用强于单用穴位埋线,可能和改善海马CA1区神经元超微结构及胆碱能系统功能有关。
[Abstract]:Objective to observe the effect of acupoint catgut implantation and insulin on learning and memory in rats with Alzheimer's disease AD and to explore its possible therapeutic mechanism. Methods A rat model of AD was established by microinjection of Okadaic acidoic acid into the CA1 region of hippocampus. Shenshu, Dazhui acupoint catgut embedding, insulin injection into lateral ventricle. The ability of learning and memory was measured by Morris water maze test, the ultrastructure of neurons in hippocampal CA1 was observed by electron microscope, and the expression of choline acetyltransferase (ChAT) was observed by immunohistochemical method. Results compared with the model group, the learning and memory ability of the combined group and the acupoint embedding group were significantly improved (P0.01), the neuronal apoptosis in the hippocampal CA1 area was significantly improved (P0.05), and the expression of chat was increased significantly in the combined group (P0.05). Conclusion the effect of acupoint catgut implantation combined with insulin on learning and memory of AD rats is stronger than that of acupoint embedding alone, which may be related to the improvement of neuronal ultrastructure and cholinergic system function in hippocampal CA1 area.
【作者单位】：贵阳医学院;
【基金】：贵州省优秀科技人才专项基金项目(No.S2001-9)
【分类号】：R245;R96

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