电针足三里和天枢穴对肠易激综合征大鼠脑肠轴不同靶点的效应差异

发布时间：2018-08-24 21:01

【摘要】：目的:比较电针足三里和天枢穴对肠易激综合征(IBS)模型大鼠内脏高敏的调节效应差异,探讨两穴作用的不同靶点,为进一步研究腧穴效应的特异性规律提供实验依据。方法:采用醋酸灌肠法制备IBS动物模型。将模型大鼠随机分为3组,即模型组、电针足三里组、电针天枢组。电针两穴组给予20min电针刺激,隔日1次,共电针治疗7次。观察电针对大鼠内脏高敏行为反应的影响,同时检测各组大鼠血液及脑肠不同靶点处的神经肽Y(NPY)含量变化。结果:与模型组比较,电针足三里组大鼠腹部抬起和背部拱起的容量阈值明显升高(P0.05)。且电针足三里组大鼠丘脑中NPY含量显著性升高(P0.05)。电针天枢组结肠组织中NPY含量明显升高(P0.05)。结论:足三里穴有直接的明显抑制内脏高敏的效应,并对丘脑中NPY有明显的调节作用。天枢穴对内脏高敏的抑制作用不明显,天枢穴只对结肠局部的脑肠肽紊乱有调节作用,推测NPY在丘脑中的含量变化可能是两穴作用差异的关键性调节物质。
[Abstract]:Objective: to compare the regulatory effects of electroacupuncture at Zusanli and Tianshu on visceral Gao Min in (IBS) rats with irritable bowel syndrome, and to explore the different targets of the two points, and to provide experimental basis for further study on the specificity of acupoint effect. Methods: IBS animal model was established by acetic acid enema. The model rats were randomly divided into three groups: model group, electroacupuncture Zusanli group and electroacupuncture Tianshu group. Electroacupuncture two points group was stimulated by 20min electroacupuncture, once every other day, 7 times electroacupuncture treatment. To observe the effect of electroacupuncture on the behavior of visceral Gao Min in rats, and to detect the changes of neuropeptide Y (NPY) content at different targets in blood and brain and intestine of rats in each group. Results: compared with the model group, the volume threshold of abdominal lift and back arch was significantly increased in Zusanli group (P0.05). The content of NPY in thalamus of Zusanli group was significantly higher than that of Zusanli group (P 0.05). The content of NPY in colon tissue of Tianshu group was significantly higher than that of control group (P 0.05). Conclusion: Zusanli point has a direct and obvious inhibitory effect on visceral Gao Min and a significant regulatory effect on NPY in thalamus. The inhibitory effect of Tianshu point on visceral Gao Min was not obvious, and Tianshu point could only regulate the brain intestinal peptide disorder in colon area. It was speculated that the change of NPY content in thalamus might be the key regulatory substance of the difference between the two points.
【作者单位】：北京中医药大学针灸推拿学院;
【基金】：国家重点基础研究发展计划(973计划)(No.2012CB508506) 北京农学院北京市重点实验室开放性课题(No.TCVM201101)~~
【分类号】：R245

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