中西医结合治疗原发性肝癌78例临床疗效和预后相关因素分析及其实验研究
发布时间：2018-10-18 18:54
【摘要】：目的:观察中西医结合治疗原发性肝癌的临床疗效,分析各种因素对预后的影响,并探讨参麦、榄香烯、复方苦参注射液对体外肝癌细胞转移相关因子的影响。方法:统计山东中医药大学附属医院和山东省临沂市中医院2000年1月至2004年2月间资料完整的住院病人和部分门诊病人,进行统计学处理。用MTT法观察三种中药注射液对肝癌细胞BEL-7402体外杀伤作用,用流式细胞仪检测对转移相关因子CD44V6和nm23等表达的影响。结果:中西医结合放化疗组和中西医结合保守治疗组对原发性肝癌患者的半年和一年生存期无明显差异;肝功能分级、门静脉瘤栓形成、临床分期、AFP和肝硬化对生存率及评价预后均有不同程度的影响和参考价值:三种中药注射液均具不同程度的体外抑瘤作用,以榄香烯体外抑瘤作用最强;并能明显抑制促转移因子CD44v6的表达、促进抑转移因子nm23的表达,且三组之间比较差异显著。结论:中西医结合是原发性肝癌的有效治疗方法,中药可通过直接抑瘤和抗转移而发挥综合抗肿瘤作用。
[Abstract]:Objective: to observe the clinical effect of integrated traditional Chinese and western medicine in the treatment of primary liver cancer, to analyze the influence of various factors on the prognosis, and to explore the effect of Shenmai, elemene and compound Sophora flavescens injection on the factors related to the metastasis of hepatocellular carcinoma cells in vitro. Methods: the inpatients and some outpatients with complete data from January 2000 to February 2004 in the affiliated Hospital of Shandong University of traditional Chinese Medicine and Linyi City Hospital of traditional Chinese Medicine of Shandong Province were statistically analyzed. MTT assay was used to observe the killing effect of three kinds of traditional Chinese medicine injection on hepatoma cell line BEL-7402 in vitro. Flow cytometry was used to detect the expression of metastasis related factors (CD44V6 and nm23). Results: there was no significant difference in the survival time of patients with primary liver cancer between radiotherapy and chemotherapy group and conservative treatment group, liver function grade, portal vein thrombus formation, liver function grade, portal vein thrombosis formation, liver function grade, portal vein thrombosis formation, liver function grade, portal vein tumor thrombus formation, Clinical staging, AFP and cirrhosis had different influence on survival rate and evaluation of prognosis. Three kinds of traditional Chinese medicine injection all had different degree of tumor inhibition in vitro, and elemene had the strongest effect in vitro. It also inhibited the expression of CD44v6 and promoted the expression of nm23, and the difference was significant among the three groups. Conclusion: integrated traditional Chinese and western medicine is an effective treatment for primary liver cancer. Chinese medicine can play a comprehensive antitumor effect through direct tumor inhibition and metastasis.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R735.7
本文编号：2280067
[Abstract]:Objective: to observe the clinical effect of integrated traditional Chinese and western medicine in the treatment of primary liver cancer, to analyze the influence of various factors on the prognosis, and to explore the effect of Shenmai, elemene and compound Sophora flavescens injection on the factors related to the metastasis of hepatocellular carcinoma cells in vitro. Methods: the inpatients and some outpatients with complete data from January 2000 to February 2004 in the affiliated Hospital of Shandong University of traditional Chinese Medicine and Linyi City Hospital of traditional Chinese Medicine of Shandong Province were statistically analyzed. MTT assay was used to observe the killing effect of three kinds of traditional Chinese medicine injection on hepatoma cell line BEL-7402 in vitro. Flow cytometry was used to detect the expression of metastasis related factors (CD44V6 and nm23). Results: there was no significant difference in the survival time of patients with primary liver cancer between radiotherapy and chemotherapy group and conservative treatment group, liver function grade, portal vein thrombus formation, liver function grade, portal vein thrombosis formation, liver function grade, portal vein thrombosis formation, liver function grade, portal vein tumor thrombus formation, Clinical staging, AFP and cirrhosis had different influence on survival rate and evaluation of prognosis. Three kinds of traditional Chinese medicine injection all had different degree of tumor inhibition in vitro, and elemene had the strongest effect in vitro. It also inhibited the expression of CD44v6 and promoted the expression of nm23, and the difference was significant among the three groups. Conclusion: integrated traditional Chinese and western medicine is an effective treatment for primary liver cancer. Chinese medicine can play a comprehensive antitumor effect through direct tumor inhibition and metastasis.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R735.7
【参考文献】
相关期刊论文 前10条
1 杨新中,李金彩,邹银水,张其军,谭晓云;对中医肿瘤病因病机与治疗的思考[J];中国医药学报;1999年06期
2 丁国善,徐冠南,付志仁,李先兴;原发性肝癌对化疗药物敏感性的研究[J];肝胆胰外科杂志;1999年04期
3 杨新中;中医药防治肿瘤转移的思路[J];湖北中医杂志;2002年08期
4 叶红军,太京华,姜英,孙述臣,肖红;原发性肝癌患者的干扰素、细胞毒性因子和肿瘤坏死因子的观察[J];临床肝胆病杂志;1997年02期
5 牛红梅,刘嘉湘;中医药抗癌转移的机理研究[J];辽宁中医杂志;2002年04期
6 闫洪飞;中医药防治肿瘤转移的探讨[J];上海中医药大学学报;2003年04期
7 刘洁,杨世杰,杨旭,陈正,李景洛;蚕蛹虫草的抗肿瘤及激素样作用[J];中国中药杂志;1997年02期
8 王国民,宋美芳,徐怀玉,费苛,沈维敏,李南麟;肝癌动脉栓塞化疗配合全肝移动条野放疗——60例前瞻性随机分组[J];中华放射肿瘤学杂志;1992年03期
9 李佩文;中药预防肿瘤转移的临床及基础研究[J];中国肿瘤;1999年01期
10 林洪生,李树奇,朴炳奎,李攻戍,裴迎霞,祁鑫;三参冲剂对肺癌转移中内皮细胞及粘附因子的影响[J];中国肿瘤;1999年12期
，本文编号：2280067
本文链接：https://www.wllwen.com/yixuelunwen/zhxiyjh/2280067.html
