中西医治疗方法对AM小鼠不同作用靶点的比较研究

发布时间：2019-01-30 08:32

【摘要】：目的以雌激素效应相关因子、血管生成相关因子及其相互作用为靶点,探讨比较中西医治疗方法对子宫腺肌病(adenomysis,AM)小鼠子宫在位及异位内膜的不同作用机制。方法对8周龄ICR雌鼠施行雄鼠垂体宫腔内移植手术,术后分别饲养3月,形成模型组。选择导师行气化瘀法临床验方(蒲灵化瘀止痛方)为实验药物(A组),孕三烯酮组为西药对照组(B组),未造模同期小鼠为空白对照组。各组分别给药3月。运用免疫组化技术观察雌激素效应相关因子(p450、COX-2、ERa)及血管生成相关因子(VEGF、CD31、MMP-2)在模型组、用药组的变化。结果在位内膜:与空白组比较,模型组CD31表达显著下降,MMP-2、VEGF显著升高;治疗后,A、B组CD31上调,A组显著优于B组,MMP-2、VEGF表达与模型组比较均显著下调,各组之间无统计学差异。异位内膜:与模型组比较,A、B组CD31表达显著下降,组间比较无统计学差异。治疗后A、B组MMP-2均显著下降,B组显著低于A组。在位及异位内膜p450、COX-2模型组显著高于空白组,治疗后p450、COX-2表达与模型组比较A、B组显著下降,组间比较无统计学差异。与模型组比较,A、B组治疗后ERa在位内膜及异位内膜显著下调,各组间比较无统计学差异。结论蒲灵化瘀止痛方可以有效改善在位内膜的缺血缺氧状态,与西药比较有明显优势,通过终止疾病的始动环节而达到治疗该病的目的。中药治疗可以有效减少异位内膜雌激素水平,疗效与西药孕三烯酮比较无明显差异。
[Abstract]:Objective to investigate the different mechanisms of ectopic and eutopic endometrium in adenomysis,AM mice by using estrogen related factors, angiogenesis related factors and their interactions as targets, and to compare the effects of traditional Chinese and western medicine on the eutopic and ectopic endometrium in mice with adenomyosis (adenomysis,AM). Methods Intrauterine pituitary transplantation was performed on 8 week old ICR female rats. The clinical test prescription (Puling Huayu Zhitong recipe), the pregnant trienone group, the western medicine control group (group B) and the control group were selected as the experimental drug (group A) and the control group (group B). Each group was given drug for 3 months. The changes of estrogen effect-related factor (p450 COX-2ERa) and angiogenic related factor (VEGF,CD31,MMP-2) in model group and drug group were observed by immunohistochemical technique. Results in eutopic endometrium, compared with blank group, the expression of CD31 in model group decreased significantly, and MMP-2,VEGF increased significantly. After treatment, CD31 in group A was up-regulated, and the expression of MMP-2,VEGF in group A was significantly lower than that in group B, but there was no statistical difference between the two groups. Ectopic endometrium: compared with the model group, the expression of CD31 decreased significantly in group A and B, but there was no significant difference between the two groups. After treatment, the MMP-2 of group A B was significantly lower than that of group A. The expression of p450 COX-2 in eutopic and ectopic endometrium in the model group was significantly higher than that in the control group. After treatment, the expression of p450 moxibustion COX-2 was significantly lower than that in the Aneb group, but there was no significant difference between the two groups. Compared with the model group, ERa eutopic endometrium and ectopic endometrium decreased significantly in group A B after treatment, but there was no significant difference between each group. Conclusion Pu Ling Huayu Zhitong prescription can effectively improve the ischemia and anoxia state of eutopic endometrium, which has obvious advantages compared with western medicine, and can be used to treat the disease by terminating the initiation of the disease. Traditional Chinese medicine can effectively reduce the level of ectopic endometrial estrogen, and there is no significant difference between the therapeutic effect and western medicine pregnancy trienone.
【作者单位】：三峡大学;成都中医药大学;
【基金】：国家自然科学基金(No.81273796)
【分类号】：R711.71

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