转录因子HSF1在食管鳞状细胞癌组织中的表达及其临床意义

发布时间：2018-01-10 17:11

本文关键词：转录因子HSF1在食管鳞状细胞癌组织中的表达及其临床意义　出处：《中国肿瘤生物治疗杂志》2015年04期 　论文类型：期刊论文

【摘要】：目的:检测热激因子1(heat-shock factor 1,HSF1)在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)组织中的表达水平,探讨其与ESCC临床病理特征及预后的关系。方法:选取2009年1月至2009年12月在山东大学齐鲁医院胸外科行手术治疗的90例ESCC患者的组织标本及50例相应癌旁组织标本,采用免疫组化法检测ESCC中HSF1蛋白的表达;另取20例2014年9月至2014年11月行手术切除的ESCC组织及癌旁组织标本,采用Real-time PCR法检测HSF1mRNA在ESCC及癌旁组织中的表达。分析HSF1蛋白表达与患者临床病理指标之间的关系,Kaplan-Meier法计算患者的5年生存率,Log-rank法比较患者的生存差别,Cox回归多因素分析判定独立的预后因素。结果:与癌旁组织相比,ESCC组织中HSF1 mRNA及蛋白的表达显著增高(均P0.01);HSF1蛋白的表达与患者年龄(P=0.453)、性别(P=0.692)、吸烟史(P=0.318)及饮酒史(P=0.367)均无显著相关性,与淋巴结转移(P=0.002)、分化程度(P=0.012)及TNM分期(P=0.024)显著相关。单因素及多因素分析结果显示,HSF1高表达与患者不良预后有关。结论:HSF1在ESCC组织中呈现高表达,与ESCC的恶性进展及不良预后密切相关。
[Abstract]:Objective: to detect heat shock factor 1. Expression of HSF1 in esophageal squamous cell carcinomas. To investigate the relationship between ESCC and clinicopathological features and prognosis. Methods:. From January 2009 to December 2009, the tissue specimens of 90 patients with ESCC and 50 specimens of adjacent tissues were collected from thoracic surgery department of Qilu Hospital, Shandong University. The expression of HSF1 protein in ESCC was detected by immunohistochemical method. From September 2014 to November 2014, 20 cases of ESCC tissues and paracancerous tissues were taken out. The expression of HSF1mRNA in ESCC and adjacent tissues was detected by Real-time PCR method, and the relationship between the expression of HSF1 protein and clinicopathological parameters was analyzed. The 5-year survival rate was calculated by Kaplan-Meier method and the survival difference was compared by Log-rank method. Results: compared with paracancerous tissues, the expression of HSF1 mRNA and protein was significantly increased (all P 0.01). There was no significant correlation between the expression of HSF1 protein and the patients' age, sex, smoking history and drinking history. There was a significant correlation between P0. 002 and P0. 012) and TNM staging (P 0. 024). The results of univariate and multivariate analysis showed that there was a significant correlation between P0. 002 and P0. 024). Conclusion the high expression of HSF1 in ESCC tissues is closely related to the malignant progression and poor prognosis of ESCC.
【作者单位】：山东大学齐鲁医院胸外科;
【基金】：国家自然科学基金资助项目(No.30571844) 山东省自然科学基金资助项目(No.ZR2013HM089) 吴阶平医学基金资助项目(No.320.6750.12393)~~
【分类号】：R735.1
【正文快照】： 世界范围内,食管癌发病率居恶性肿瘤的第六位,病死率居第八位[1]。我国是食管癌高发国家,其中90%以上是鳞状细胞癌[2]。食管癌早期无明显症状,发病原因不确切,临床上发现时多属于中、晚期,治疗效果欠佳[3]。多种分子标志物可用于食管鳞状细胞癌(esophageal squamous cell carc

【参考文献】