紧密连接相关蛋白在肺癌脑转移瘤中的表达及临床意义

发布时间：2018-01-13 01:33

本文关键词：紧密连接相关蛋白在肺癌脑转移瘤中的表达及临床意义　出处：《南昌大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:探讨血-脑屏障Claudin-5、Occludin及Zo-1蛋白在肺癌脑转移瘤组织和正常脑组织中的表达差异,并分析其临床意义。方法:应用Envision免疫组化法,检测Claudin-5、Occludin及Zo-1在63例肺癌脑转移瘤组织和28例正常脑组织中的表达情况,研究三种紧密连接蛋白在转移瘤组织和正常组织中的不同表达,并应用SPSS软件,分析转移瘤中三种紧密连接蛋白表达水平与患者各种临床病理因素(性别、年龄、转移灶大小及个数、病理分型)、生存时间的关系。结果:1.Claudin-5蛋白主要表达于细胞膜中,其在正常脑组织、脑转移瘤组织中的高表达率各为50%和25.4%,差异有统计学意义(χ2=5.310,P=0.021)。Claudin-5在肺癌脑转移瘤病灶中的表达与患者的年龄及转移灶个数有关,差异有统计学意义,而与患者性别、肿块直径、原发灶病理学分型无统计学相关性。2.Occludin蛋白主要表达在细胞膜中,在正常脑组织、转移瘤组织中的高表达率各为57.1%、34.9%,差异有统计学意义(χ2=3.936,P=0.047)。脑转移瘤组织中Occludin的表达与患者的年龄及转移灶个数有关,与患者性别、肿块直径、原发灶病理学分型无关,差别无统计学意义(P0.05)。3.胞质附属蛋白Zo-1在正常脑组织、转移瘤组织中的高表达率各为75%、33.3%,两者高表达率的差异有统计学意义(χ2=13.542,P0.001)。肺癌脑转移瘤组织中Zo-1的表达与患者的年龄及转移灶个数有关(P0.05),差别有统计学意义,而与患者性别、肿块直径、原发灶病理学分型无明显相关性。4.Claudin-5、Occludin及Zo-1蛋白在高表达组的mOS较低表达组的mOS长,差别有统计学意义(P0.05)。5.采用Spearman相关分析提示,Claudin-5、Occludin及Zo-1在肺癌脑转移瘤组织中的表达,两两之间呈正相关(P0.05)。结论:与正常脑组织相比,血-脑屏障上的Claudin-5、Occludin及Zo-1蛋白在脑转移瘤组织中呈低表达,表达水平的高低与患者的生存时间有关,提示紧密连接相关蛋白Claudin-5、Occludin、Zo-1表达下调在肺癌脑转移瘤的发生发展中可能起着一定的作用。
[Abstract]:Objective: to investigate the expression of Claudin-5 Occludin and Zo-1 protein in brain metastases of lung cancer and normal brain tissues. Methods: Envision immunohistochemical method was used to detect Claudin-5. The expression of Occludin and Zo-1 in 63 cases of lung cancer brain metastases and 28 cases of normal brain tissues were studied. SPSS software was used to analyze the expression level of three kinds of tight junction protein in metastatic tumor and the clinicopathological factors (sex, age, size and number of metastatic foci, pathological classification). Results 1. Claudin-5 protein was mainly expressed in cell membrane. The high expression rates of Claudin-5 protein in normal brain tissues and brain metastases were 50% and 25.4% respectively. The expression of Claudin-5 in brain metastases of lung cancer was related to the age of patients and the number of metastatic foci. The difference was statistically significant, but there was no statistical correlation with sex, tumor diameter and pathological classification of primary lesion. 2.Occludin protein was mainly expressed in the cell membrane and normal brain tissue. The high expression rates in metastatic tumor tissues were 57.1 and 34.9respectively, the difference being statistically significant (蠂 2 / 3.936). The expression of Occludin in brain metastases was related to the age of patients and the number of metastatic foci, but not to sex, tumor diameter and pathological type of primary tumor. The high expression rate of cytoplasmic accessory protein Zo-1 in normal brain tissue and metastatic tumor tissue was 75% and 33 3% respectively. The difference of high expression rate between the two groups was statistically significant (蠂 2 = 13.542). The expression of Zo-1 in brain metastases of lung cancer was related to the age of patients and the number of metastatic foci. The difference was statistically significant, but it was related to the sex of patients and the diameter of tumor. There was no significant correlation between pathological classification of primary lesions. 4. Claudin-5 Occludin and Zo-1 protein were longer in mOS with high expression than those with low expression. The difference was statistically significant (P 0.05). Spearman correlation analysis suggested that Claudin-5. The expression of Occludin and Zo-1 in brain metastases of lung cancer was positively correlated with that of normal brain tissue (P 0.05). The expression of Claudin-5 cludin and Zo-1 protein in the blood-brain barrier was low in the brain metastases, and the expression level was related to the survival time of the patients. It is suggested that the down-regulation of the tight junction associated protein Claudin-5 Occludingnan Zo-1 may play a role in the development of brain metastases of lung cancer.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R734.2

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