β-catenin、MUC1表达与食管癌发生发展的相关性研究

发布时间：2018-01-26 05:27

本文关键词： MUC1 β-catenin 食管鳞癌　出处：《川北医学院》2017年硕士论文　论文类型：学位论文

【摘要】：目的:通过检测β-连环蛋白(β-catenin,β-cat)、粘蛋白1(mucin1,MUC1)在正常食管粘膜、食管粘膜上皮内瘤变及不同临床分期食管鳞癌中的表达,探讨β-catenin、MUC1与食管癌发生发展的相关性,从而为食管癌早期诊断、推测预后提供参考;并为探索食管癌治疗的新靶点提供初步的理论依据。方法:采用免疫组织化学染色检测正常食管粘膜、食管粘膜上皮内瘤变及不同临床分期食管鳞癌中β-catenin、MUC1的表达水平。用SPSS13.0统计学软件进行统计学分析。结果:1.β-catenin在正常食管粘膜、食管粘膜上皮内瘤变、食管癌中阳性表达率逐渐升高,差异具有统计学意义(P0.05);低级别上皮内瘤变组阳性表达率显著高于正常食管粘膜组(P0.05);低级别上皮内瘤变组与高级别上皮内瘤变组间、食管粘膜瘤样变组与食管癌组间差异无统计学意义(P0.05)。在食管癌组中,肿瘤≥3cm组阳性表达率显著高于肿瘤3cm组,累及肌层和外膜层组阳性表达率显著高于局限粘膜及粘膜下层组,有淋巴结转移组阳性表达率显著高于无淋巴结转移组,肿瘤分期III+IV组阳性表达率显著高于肿瘤I+II组,差异均有统计学意义(P0.05)。2.MUC1在正常食管粘膜、食管粘膜上皮内瘤变、食管癌中阳性表达率逐渐升高,差异具有统计学意义(P0.05);高级别上皮内瘤变组阳性表达率显著高于正常食管粘膜组(P0.05);低级别上皮内瘤变组与正常食管粘膜组间、低级别上皮内瘤变组与高级别上皮内瘤变组间、食管粘膜瘤样变组与食管癌组间差异无统计学意义(p0.05)。在食管癌组中,肿瘤≥3cm组阳性表达率显著高于肿瘤3cm组,累及肌层和外膜层组阳性表达率显著高于局限粘膜及粘膜下层组,有淋巴结转移组阳性表达率显著高于无淋巴结转移组,肿瘤分期iii+iv组阳性表达率显著高于肿瘤i+ii组,差异均有统计学意义(p0.05)。3.在正常食管粘膜组、食管粘膜上皮内瘤变组、食管癌组中,β-catenin、muc1共同阳性表达率逐渐升高,差异具有统计学意义(p0.05),低级别上皮内瘤变组共同阳性表达率显著高于正常食管粘膜组(p0.05),低级别上皮内瘤变组与高级别上皮内瘤变组间、食管粘膜瘤样变组与食管癌组间差异无统计学意义(p0.05)。β-catenin、muc1在正常食管粘膜组、食管粘膜上皮内瘤变、食管癌组中呈正相关(p0.01)。在食管癌组,β-catenin、muc1共同阳性表达率在累及肌层和外膜层组阳性表达率显著高于局限粘膜及粘膜下层组,有淋巴结转移组阳性表达率显著高于无淋巴结转移组,肿瘤分期iii+iv组阳性表达率显著高于肿瘤i+ii组,差异均有统计学意义(p0.05)。结论:1.β-catenin、muc1的异常表达与食管粘膜上皮内瘤变及食管癌的发生密切相关,可能是食管癌发生的重要分子机制之一。2.在食管粘膜上皮内瘤变中,β-catenin、muc1异常表达显著高于正常食管粘膜,二者作为分子标记,对食管粘膜癌前病变的早期筛查可能有潜在的临床应用价值。3.β-catenin、muc1的阳性表达与肿瘤大小、肿瘤分期、浸润程度、淋巴结转移关系密切。提示β-catenin、muc1与肿瘤的转移、侵袭有关,对判断食管癌的预后有一定的价值。4.β-catenin、MUC1的共同阳性表达率在食管粘膜瘤样病变及食管癌组织中明显高于正常食管粘膜,二者在食管癌的发生发展中可能起协同作用。针对β-catenin、MUC1的基因或免疫治疗或许是食管癌治疗的新靶点,值得进一步研究。
[Abstract]:Objective: through the detection of beta catenin (-catenin beta, beta -cat), mucin 1 (Mucin1, MUC1) in normal esophageal mucosa, the expression of esophageal intraepithelial neoplasia and esophageal squamous cell carcinoma in different clinical stages, to explore the development of beta -catenin, relationship between MUC1 and esophageal cancer, and early diagnosis of esophageal cancer the prognosis, to provide reference; and provide the theoretical basis for the exploration of new targets for treatment of esophageal carcinoma. Methods: immunohistochemical staining of normal esophageal mucosa, esophageal intraepithelial neoplasia and esophageal squamous cell carcinoma in different clinical stages of beta -catenin, the expression level of MUC1. Statistical analysis was performed by SPSS13.0 statistical software. 1.: beta -catenin in normal esophageal mucosa, esophageal intraepithelial neoplasia, the positive expression rate of esophageal cancer is gradually increased, the difference was statistically significant (P0.05); low grade intraepithelial neoplasia group significant positive expression rate Higher than normal esophageal mucosa group (P0.05); low-grade intraepithelial neoplasia and high-grade intraepithelial neoplasia groups, esophageal mucosal neoplasia and esophageal cancer. The difference between groups was not statistically significant (P0.05). In esophageal carcinoma group, tumor group than 3cm positive expression rate was significantly higher than that of tumor 3cm group. Involving the muscular layer and adventitia group positive expression rate was significantly higher than that of the limitations of the mucosa and submucosa group, the group with lymph node metastasis positive expression rate was significantly higher than that in the group without lymph node metastasis, tumor stage III+IV group positive expression rate was significantly higher than that of tumor in I+II group, there were statistically significant differences (P0.05).2.MUC1 in normal esophageal mucosa and esophageal mucosa intraepithelial neoplasia, the positive expression rate of esophageal cancer is gradually increased, the difference was statistically significant (P0.05); high grade intraepithelial neoplasia group positive expression rate was significantly higher than that in normal esophageal mucosa group (P0.05); low grade intraepithelial neoplasia group and normal. Tube mucosa group, low-grade intraepithelial neoplasia and high-grade intraepithelial neoplasia groups, esophageal mucosal neoplasia and esophageal cancer. The difference between groups was not statistically significant (P0.05). In esophageal carcinoma group, tumor group than 3cm positive expression rate was significantly higher than that of tumor group 3cm, involving the muscular layer and the outer layer group positive expression rate was significantly higher than that of the limitations of the mucosa and submucosa group, the group with lymph node metastasis positive expression rate was significantly higher than that in the group without lymph node metastasis, tumor stage iii+iv group positive expression rate was significantly higher than that of tumor in i+ii group, there were statistically significant differences (P0.05).3. in normal esophageal mucosa, mucosa of esophageal intraepithelial neoplasia group -catenin beta esophageal cancer group, MUC1, positive expression rate gradually increased, the difference was statistically significant (P0.05), low grade intraepithelial neoplasia group positive expression rate was significantly higher than that in normal esophageal mucosa group (P0.05), low grade intraepithelial neoplasia The group with high grade intraepithelial neoplasia group, esophageal mucosal neoplasia and esophageal cancer. The difference between groups was not statistically significant (P0.05). Beta -catenin, MUC1 in normal esophageal mucosa, esophageal intraepithelial neoplasia and esophageal cancer was positively related to group (P0.01). In the esophageal cancer group, P -catenin MUC1, positive expression rate in the involved muscle layer and adventitia group positive expression rate was significantly higher than that of the limitations of the mucosa and submucosa group, the group with lymph node metastasis positive expression rate was significantly higher than that in the group without lymph node metastasis, tumor stage iii+iv group positive expression rate was significantly higher than that of tumor i+ II group, the differences were statistically significant (P0.05). Conclusion: the abnormal expression of -catenin beta 1., MUC1 and esophageal epithelial neoplasia and esophageal cancer is closely related, may be an important molecular mechanism of esophageal cancer.2. in esophageal mucosa intraepithelial neoplasia, beta -catenin, abnormal expression of MUC1 was significantly higher In normal esophageal mucosa, two as molecular markers, may have clinical application value of.3. beta -catenin potential for early diagnosis of esophageal mucosa precancerous lesions, the positive expression of MUC1 with tumor size, tumor stage, tumor invasion, lymph node metastasis. It is suggested that beta -catenin, metastasis, MUC1 and tumor invasion. Have a certain value of.4. beta -catenin in prognosis of esophageal cancer, a common positive rate of expression of MUC1 in tissue of esophageal mucosa neoplasia and esophageal cancer was significantly higher than that of normal esophageal mucosa, two in esophageal carcinoma development may play a synergistic effect. The beta -catenin gene or MUC1 immune therapy may be a new target for the treatment of esophageal cancer, it is worthy of further study.

【学位授予单位】：川北医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.1

【参考文献】