细胞周期相关蛋白在人结肠癌组织中的表达及其临床意义的探讨

发布时间：2018-01-26 16:15

本文关键词： 结肠癌 NIK-IKK结合蛋白周期蛋白E 周期蛋白D1　出处：《广西医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:检测结肠癌患者组织中细胞周期蛋白D1(cyclin D1)、细胞周期蛋白E(cyclin E)以及NIK-IKK结合蛋白(NIBP)的表达,探讨其与临床、病理特征之间的关系,并分析三者在结肠癌组织中表达的相关性。为结肠癌的早期诊断和预后提供一个可靠的分子生物学指标。方法:收集从2015年2月到2015年12月之间广西医科大学第一附属医院结直肠外科经外科手术切除及消化内科肠镜活检的部分标本130例,正常结肠黏膜、结肠癌各65例,所有的患者均未接受放化疗。采用免疫组织化学SP法分别检测并分析NIBP、cyclin E和cyclinD1的表达和相关性,并分析上述蛋白的表达与结肠癌患者肿瘤的浸润深度、TNM临床分期、淋巴结转移和远处转移相关性。结果:(1)NIBP在结肠癌组织中高表达阳性率为60.00%(39/65),明显高于正常结肠粘膜组织中的阳性表达率[18.46%(12/65),P0.05],差异具有统计学意义;Cyclin E在结肠癌组织中高表达阳性率为56.92%(37/65),显著高于正常结肠粘膜组织中的阳性表达率[3.08%(2/65),P0.05],差异具有统计学意义;CyclinD1在结肠癌组织中高表达阳性率为89.23%(58/65),显著高于正常结肠粘膜组织中的阳性表达率[43.08%(28/65),P0.05],差异具有统计学意义。(2)结肠癌组织中CyclinE、NIBP和Cyclin D1的表达与肿瘤浸润深度、TNM临床分期、淋巴结转移和远处转移明显相关(p0.05)。(3)结肠癌组织中NIBP和Cyclin D1表达之间呈正相(r=0.32,P0.05),NIBP和CyclinE表达之间呈正相关(r=0.43,P0.05),CyclinD1和CyclinE表达之间呈正相关(r=0.23,P0.05)。结论:(1)NIBP、Cyclin E、Cyclin D1在结肠癌中高表达,三者可能参与了结肠癌的发生发展;(2)三者在结肠癌中的高表达都可促进肿瘤细胞的浸润与转移,其表达越高,浸润深度越深,淋巴结及远处转移可能性越大。(3)NIBP可能促进CyclinE和Cyclin D1的高表达而导致细胞周期调控异常,三者可能共同参与了结肠癌的发生发展。
[Abstract]:Objective: to detect cyclin D1 cyclin D1 in the tissues of colon cancer patients. The expression of cyclin E (cyclin E) and NIK-IKK binding protein (NIBP) and its relationship with clinical and pathological features were investigated. The correlation of the expression of the three proteins in colon cancer tissues was analyzed to provide a reliable molecular biological index for the early diagnosis and prognosis of colon cancer. Methods:. A total of 130 specimens were collected from the first affiliated Hospital of Guangxi Medical University from February 2015 to December 2015. 65 cases of normal colon mucosa and 65 cases of colon cancer, all patients did not receive radiotherapy and chemotherapy. Immunohistochemical SP method was used to detect and analyze NIBP. The expression and correlation of cyclin E and cyclinD1, and the relationship between the expression of these proteins and the depth of tumor invasion in patients with colon cancer were analyzed. Results the positive rate of high expression of NIBP in colon cancer tissues was 60.009 / 65). The positive expression rate in normal colonic mucosa was significantly higher than that in normal colonic mucosa. [18.46% / 65% P 0.05, the difference was statistically significant; The positive rate of high expression of Cyclin E in colon cancer tissues was 56.92% 37 / 65, which was significantly higher than that in normal colonic mucosa tissues. [The difference was statistically significant (P 0.05). The high expression rate of CyclinD1 in colon cancer tissue was 89.23%, which was significantly higher than that in normal colonic mucosa tissue. [43.08% 28% 65% (P0.05), the difference was statistically significant (P < 0.05) CyclinE in colon cancer tissue. The expression of NIBP and Cyclin D1 was correlated with the depth of tumor invasion. Lymph node metastasis and distant metastasis were significantly correlated with the expression of NIBP and Cyclin D1 in colorectal cancer tissues. There was a positive correlation between the expression of NIBP and CyclinE and the expression of cyclin D1 and CyclinE. Conclusion the high expression of Cyclin E Cyclin D1 in colon cancer may be involved in the occurrence and development of colon cancer. (2) the high expression of these three proteins in colon cancer can promote the invasion and metastasis of tumor cells, the higher the expression, the deeper the depth of invasion. The higher the possibility of lymph node and distant metastasis, the higher the expression of CyclinE and Cyclin D1, which may lead to abnormal cell cycle regulation. The three may be involved in the occurrence and development of colon cancer.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.35

【相似文献】