网膜囊冲洗液检测在胃癌诊治中的应用价值

发布时间：2018-01-28 01:06

本文关键词： 胃肿瘤网膜囊冲洗液流式细胞术腹腔冲洗液细胞学　出处：《世界华人消化杂志》2015年07期 　论文类型：期刊论文

【摘要】：目的:初步评估胃癌患者术中网膜囊冲洗液检测的应用价值.方法:分析78例胃癌患者的临床资料,分别收集术中网膜囊冲洗液,采用流式细胞术(flow cytometry,FCM)检测网膜囊冲洗液中游离细胞的癌胚抗原(carcino-embryonic antigen,CEA)表达情况,同时行腹腔冲洗液细胞学(peritoneal lavage cytology,PLC)检查.结果:网膜囊冲洗液FCM和PLC的检测阳性率分别为37.1%(29/78)、11.5%(9/78),两者差异有统计学意义(P=0.001).胃癌网膜囊冲洗液阳性与肿瘤大小、肿瘤部位以及浸润深度密切相关(P0.05),而与年龄、性别、分化程度、脉管癌栓、侵犯面积无关(P0.05).结论:FCM可作为网膜囊冲洗液的快速检测手段,网膜囊冲洗液检测可评估肿瘤的进展情况,对预测腹膜微转移有一定的价值.
[Abstract]:Objective: to evaluate the clinical value of omentum sac flushing fluid in patients with gastric cancer. Methods: the clinical data of 78 patients with gastric cancer were analyzed and the intraoperative omentum sac flushing fluids were collected. Flow cytometry was used to flow cytometry. The expression of carcinoembryonic antigen carcinoembryonic antigen (CEA) in the flushing fluid of omentum sac was detected by FCM. Peritoneal lavage fluid lavage cytology was also performed. Results: the positive rates of FCM and PLC in the flushing fluid of omentum sac were 37.1% and 29.78% (11.5% / 78), respectively. The difference was statistically significant (P < 0.01). There was a close correlation between the flushing fluid of omentum sac of gastric cancer and tumor size, tumor location and depth of invasion (P 0.05), but with age and sex. Conclusion: FCM can be used as a rapid method for the detection of omental sac flushing fluid, and omentum sac lavage fluid can be used to evaluate the progress of tumor. It has certain value in predicting peritoneal micrometastasis.
【作者单位】：南昌大学第一附属医院普外科;南昌大学研究生院;江西省人民医院;
【基金】：江西省科技厅支撑计划基金资助项目,No.20132BBG70054~~
【分类号】：R735.2
【正文快照】： ■背景资料手术是当前治疗进展期胃癌最有效的手段,而腹膜微转移则是影响胃癌术后预后的一个重要的因素,准确评估胃癌的腹膜微转移具有重要意义.林良庆,曹毅,揭志刚,刘逸,李正荣,南昌大学第一附属医院普外科江西省南昌市330006林良庆,张国阳,南昌大学研究生院江西省南昌市3300

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