长非编码RNA BANCR逆转非小细胞肺癌细胞株顺铂耐药的研究

发布时间：2018-01-29 05:34

  本文关键词： 非小细胞肺癌 BANCR 顺铂耐药性　出处：《南京医科大学学报(自然科学版)》2017年02期 　论文类型：期刊论文

【摘要】：目的:研究长非编码RNA BANCR对人非小细胞肺癌耐顺铂细胞株A549/DDP耐药性的影响及其可能的作用机制。方法:应用实时荧光定量PCR法(q RT-PCR)检测耐顺铂细胞株A549/DDP及其亲本细胞株A549中BANCR的表达差异,发现BANCR在A549/DDP细胞中显著低表达。在A549/DDP细胞中过表达BANCR,分别应用MTT法、克隆形成实验、流式细胞术检测过表达后A549/DDP细胞对顺铂药物敏感性,细胞增殖能力,联合顺铂处理后细胞凋亡变化;Western blot检测过表达BANCR后A549/DDP细胞p53的表达变化。结果:BANCR在A549/DDP细胞中的表达量显著低于A549细胞(P0.05),在A549/DDP细胞中过表达BANCR可产生以下效应:相较于对照组,顺铂对A549/DDP/BANCR细胞的半数抑制浓度(half inhibition concentration,IC50)减低(P0.05),A549/DDP/BANCR细胞的增殖能力减弱,A549/DDP/BANCR经过顺铂处理后细胞凋亡增多(P0.05)。Western blot结果显示,与空白对照组比较,过表达BANCR的A549/DDP细胞p53表达水平明显升高。结论:BANCR可能通过诱导细胞凋亡,抑制细胞增殖,上调p53蛋白表达而逆转A549/DDP细胞对DDP的耐药性。
[Abstract]:Objective: to study the effect of long non-coding RNA BANCR on drug resistance of cisplatin resistant human non-small cell lung cancer cell line A549 / DDP and its possible mechanism. Real-time fluorescence quantitative PCR method (. The expression of BANCR in cisplatin resistant A549 / DDP cell line and its parent A549 cell line was detected by Q RT-PCR. It was found that BANCR was significantly underexpressed in A549 / DDP cells, and BANCRs were overexpressed in A549 / DDP cells. MTT assay was used to clone the BANCRs in A549 / DDP cells. Flow cytometry was used to detect the sensitivity of A549% DDP cells to cisplatin, cell proliferation and apoptosis after treatment with cisplatin. The expression of p53 in A549 / DDP cells was detected by Western blot after BANCR expression. Results:. The expression of BANCR in A549 / DDP cells was significantly lower than that in A549 cells (. P0.05). Overexpression of BANCR in A549 / DDP cells had the following effects: compared with the control group. Half of the inhibitory concentration of cisplatin on A549 / DDP / BANCR cells was half inhibition concentration. IC50) reduced the proliferation ability of P0.05A549 / DDP / BANCR cells. Apoptosis of A549 / DDP / BANCR was increased after cisplatin treatment. The results of Western blot showed that the apoptosis of A549 / DDP / BANCR was higher than that of control group. The expression of p53 in A549 / DDP cells overexpression of BANCR was significantly increased. Conclusion: BANCR may inhibit cell proliferation by inducing cell apoptosis. The expression of p53 protein was upregulated and the drug resistance of A 549% DDP cells to DDP was reversed.
【作者单位】： 南京医科大学第二附属医院肿瘤科;海门市人民医院肿瘤科;
【基金】：国家自然科学基金(81272601,81472198) 江苏省科技厅临床医学科技专项(BL2014096) 江苏省医学重点人才基金(RC2011080)
【分类号】：R734.2
【正文快照】： [Acta Univ Med Nanjing,2017,37(02):189-193]*通信作者(Corresponding author),E-mail:zhaoxiawang88@hotmail.com肺癌是世界范围内最常见恶性肿瘤之一,非小细胞肺癌(NSCLC)约占肺癌总数的80%以上[1],超过65%的患者为中晚期[2]。含铂类方案为NSCLC化疗重要的方案之一,而顺铂，

本文编号：1472672