紫檀芪对舌鳞癌细胞株Tca8113凋亡的影响研究

发布时间：2018-01-30 00:53

本文关键词： 紫檀芪 Tca8113 凋亡　出处：《锦州医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的本实验以舌鳞癌细胞系Tac8113细胞为研究对象,通过体外实验研究,从细胞水平观察紫檀芪对Tac8113细胞系的增殖抑制作用,探讨其可能存在的机制,为紫檀芪的抗舌鳞癌作用提供理论及实验依据。方法MTT方法检测紫檀芪不同浓度以及不同作用时间对舌鳞癌细胞株Tca8113生长活性的影响,使用0、2.5、5、10、15和20μmol/L的紫檀芪为终浓度的培养基培养舌鳞癌细胞株Tca8113,使用96孔板进行铺板,分别作用24h、48h和72h后上机检测OD值。用药后48h流式细胞术检测紫檀芪处理后Tca8113细胞的凋亡情况。Western blot测定Bax、Fas、Caspase3基因蛋白表达水平。使用SPSS19.0软件分析,计量资料以(?)表示,组间比较使用方差分析。P0.05为差异有统计学意义。结果MTT检测结果表明不同浓度的紫檀芪对舌鳞癌细胞株Tca8113生长有抑制作用,且随着紫檀芪药物浓度的升高,促进凋亡作用逐渐增强。形态学观察发现紫檀芪作用后的细胞呈明显凋亡状态,部分细胞胞浆浓缩特征。流式细胞仪检测表明,紫檀芪作用于舌鳞癌细胞株Tca8113,不同浓度的紫檀芪终浓度培养基培养48h,诱导细胞株总凋亡率的均值与阴性对照比较差异有统计学意义(P0.05),且随着紫檀芪药物浓度的升高,促进凋亡作用逐渐增强,2.5μmol/L紫檀芪终浓度培养基培养48h,诱导细胞株总凋亡率的均值为26.76%,20μmol/L紫檀芪共培养48h,诱导细胞株总凋亡率的均值为91.11%。Western blot测定显示,Bax、Fas、Caspase3蛋白表达增强的程度随着紫檀芪的浓度增加而升高。结论紫檀芪能抑制舌鳞癌细胞Tca8113的体外生长,上调Bax、Fas、Caspase3蛋白表达诱导其凋亡,可能是其作用机制之一。
[Abstract]:Objective to investigate the inhibitory effect of Radix Astragali on the proliferation of Tac8113 cell line, a tongue squamous cell carcinoma cell line, at the cell level. The possible mechanism of its existence is discussed. Methods MTT method was used to detect the effects of different concentrations and different time of action on the growth activity of Tca8113 cell line in tongue squamous cell carcinoma cell line. Tca8113 cell line of tongue squamous cell carcinoma was cultured on the culture medium of Astragalus membranaceus at the final concentration of 0 ~ 2. 5 and 20 渭 mol/L, and was paved with 96 well plates. The cells were treated for 24 hours respectively. OD value was detected after 48 h and 72 h, apoptosis of Tca8113 cells was detected by flow cytometry 48 h after treatment. Bax was detected by Western blot. The expression level of caspase3 gene was analyzed by SPSS19.0 software. ). Results the results of MTT test showed that different concentrations of Radix Astragali could inhibit the growth of tongue squamous cell carcinoma cell line Tca8113. With the increase of drug concentration of Radix Astragali, the effect of promoting apoptosis was gradually enhanced. Morphological observation showed that the cells treated with Rhizoma Astragali showed obvious apoptotic state, and some of the cells were concentrated in cytoplasm. Flow cytometry showed that. Radix Astragali was applied to tongue squamous cell carcinoma cell line Tca8113 and cultured on different concentration medium for 48 h. Compared with the negative control, the mean value of the total apoptosis rate of the induced cell line was significantly different from that of the negative control group (P 0.05), and with the increase of the drug concentration of Astragalus membranaceus, the effect of promoting apoptosis was gradually enhanced. The average rate of apoptosis induced by 2.5 渭 mol/L rosewood final concentration culture medium for 48 h was 26.76 渭 mol/L and 20 渭 mol/L respectively. The average apoptotic rate was 91.11%. Western blot assay showed that Baxanthus FAS. Conclusion Radix Astragali can inhibit the growth of tongue squamous cell Tca8113 in vitro and up-regulate the expression of Baxanthus FAS. The expression of Caspase3 protein induces its apoptosis, which may be one of its mechanisms.
【学位授予单位】：锦州医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.8

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