AE-PDT对裸鼠舌鳞癌治疗效果的初步探讨

发布时间：2018-02-02 00:36

本文关键词： 芦荟大黄素光动力疗法舌鳞癌裸鼠　出处：《口腔医学研究》2017年12期 　论文类型：期刊论文

【摘要】：目的:探讨芦荟大黄素(aloe-emodin,AE)介导的光动力疗法(photodynamic therapy,PDT)对人舌鳞癌治疗的疗效,为临床提供理论依据。方法:将人舌鳞癌Tca-8113细胞植入裸鼠皮下,随机分4组(对照组、光照组、AE组和PDT组),每组9只。治疗后定期测量肿瘤体积,14d后各组处死3只,剖瘤称重计算抑瘤率;并行病理形态学观察;其余6只观察生存时间,计算生命延长率。结果:各处理组肿瘤体积均小于对照组,PDT组肿瘤体积变化明显(P0.05);光照组、AE组、PDT组抑瘤率分别为:4.76%、20.24%、77.40%,PDT组抑瘤效果明显优于其他组;HE染色可见PDT组肿瘤细胞坏死及空泡变性,对照组及光照组无明显改变;各处理组均能延长小鼠生存时间,但以PDT组生存期最长、生命延长率最高。结论:AE-PDT对舌鳞癌肿瘤的生长具有抑制作用,并可延长肿瘤小鼠的生存时间。
[Abstract]:Objective: to investigate photodynamic therapy mediated by aloe emodin. Methods: human tongue squamous cell carcinoma (Tca-8113) cells were implanted subcutaneously into nude mice and randomly divided into 4 groups (control group, illumination group). The tumor volume of each group was measured 14 days after treatment, 3 rats in each group were killed and the tumor inhibition rate was calculated by tumor weight. The histopathology was observed. Results: the tumor volume of each treatment group was smaller than that of the control group (P 0.05). The tumor inhibition rate of PDT group in light group was 77.40% and the inhibition rate of PDT group was significantly better than that of other groups. He staining showed necrosis and vacuolar degeneration of tumor cells in PDT group, but no obvious change in control group and illumination group. All the treatment groups could prolong the survival time of mice, but the survival time of PDT group was the longest and the life prolongation rate was the highest. Conclusion: AE-PDT can inhibit the growth of tongue squamous cell carcinoma. It can prolong the survival time of tumor mice.
【作者单位】：哈尔滨医科大学附属第四医院口腔科;
【基金】：哈尔滨市科技局应用技术研究及开发项目(编号:2014RFXGJ095)
【分类号】：R739.86
【正文快照】： 舌鳞癌是最常见的口腔癌,具有生长快,浸润性强等特点[1,2],其治疗方式主要以手术、放疗为主,且伴明显的副作用。据统计口腔癌5年生存率仅在50%~60%。因此,寻求一种新型治疗方式尤为重要。PDT是一种新型治疗恶性肿瘤的方法,它是借助光敏剂在特定的激光光源照射下产生单态氧和自

【参考文献】