TRPV3在肝脏肿瘤中的表达及其与肝癌发展关系的研究

发布时间：2018-02-02 13:29

本文关键词： TRPV3 肝血管瘤肝细胞癌肝硬化精准医学　出处：《山西医科大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:肝细胞癌作为最常见的恶性肿瘤之一,危害人民健康和生命安全。因此探索肝细胞癌增殖发展及侵袭转移的生物学机制显得尤为重要,本实验旨在探索TRPV3在肝脏肿瘤中的表达情况及其与肝癌发展存在何种关系。方法:收集自2010年8月至2015年9月山西医科大学第一医院行肝脏疾病手术的病理标本及临床资料。用免疫组化学方法研究TRPV3在不同肝脏疾病中的表达情况,并用SPSS软件分析其与临床特征的关系。结果:97例病理标本行免疫组化实验,其中肝硬化63例,高/中表达占71.4%(45/63);肝细胞癌15例,高/中表达占60%(9/15);肝血管瘤17例,高/中表达占17.6%(3/17)。TRPV3在肝细胞癌和肝硬化中的表达无统计学意义(P=0.582)。TRPV3在肝细胞癌和肝血管瘤的表达差异有统计学意义(P=0.027)。TRPV3在肝硬化和肝血管瘤的表达差异有统计学意义(P0.001)。临床病例资料共收集58例,提示TRPV3与肿瘤大小、分化程度、病人年龄、性别、BMI无明显统计学相关。结论:TRPV3在肝血管瘤、肝细胞癌、肝硬化中有着不同程度的表达,且在肝脏良恶性肿瘤的表达差异有统计学意义。提示TRPV3的高表达可能推进了肝脏恶性疾病的发生发展,未来通过抑制TRPV3的表达可能预防肝硬化和肝癌的发生发展,但仍然需要更多的样本和基因组学方法来进一步研究TRPV3在肝细胞癌中发挥的作用。
[Abstract]:Objective: as one of the most common malignant tumors, hepatocellular carcinoma endangers people's health and life safety. Therefore, it is very important to explore the biological mechanism of proliferation, development, invasion and metastasis of hepatocellular carcinoma. The purpose of this study was to investigate the expression of TRPV3 in liver tumors and its relationship with the development of liver cancer. Methods:. From August 2010 to September 2015, pathological specimens and clinical data of liver diseases in the first Hospital of Shanxi Medical University were collected. Immunohistochemical study of TRPV3 in different liver diseases. Express the situation. SPSS software was used to analyze its relationship with clinical features. Results Immunohistochemistry was performed in 97 cases of pathological specimens, 63 of them were cirrhosis, and 71.4% / 45 / 63% of them had high / moderate expression. There were 15 cases of hepatocellular carcinoma, with high / medium expression accounting for 60% / 15%; 17 cases of hepatic hemangioma. There was no significant difference in the expression of TRPV3 in hepatocellular carcinoma and liver cirrhosis (P < 0. 582). There was significant difference in the expression of TRPV3 between hepatocellular carcinoma and hepatic hemangioma. There was a significant difference in the expression of TRPV3 in liver cirrhosis and hepatic hemangioma (P 0.001). 58 cases were collected. The results suggest that there is no significant correlation between TRPV3 and tumor size, differentiation degree, age and sex of patients. Conclusion: TRP _ 3 is associated with hepatic hemangioma and hepatocellular carcinoma. The high expression of TRPV3 may promote the occurrence and development of liver malignant diseases. Inhibition of TRPV3 expression may prevent the occurrence and development of liver cirrhosis and liver cancer in the future, but more samples and genomics methods are needed to further study the role of TRPV3 in hepatocellular carcinoma.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.7

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