原代胃癌干细胞CD44表达意义探讨

发布时间：2018-02-02 14:40

本文关键词： 胃癌肿瘤干细胞 CD 球形体　出处：《中华肿瘤防治杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的肿瘤干细胞(cancer stem cells,CSCs)在恶性肿瘤的发生发展中起着重要作用,且是肿瘤耐药及复发的根源。本研究探讨CSCs表面标记蛋白CD44在分离及鉴定胃癌干细胞中的重要作用。方法收集2015-11-06-2015-12-01华中科技大学同济医学院附属同济医院3例胃癌患者手术标本。首先采用胶原酶消化法获取胃癌细胞,部分细胞分别贴壁及悬浮培养,并采用免疫荧光及流式分析的方法检测胃癌球形体中CD44的表达情况。部分细胞采用流式细胞仪分选得到CD44~+及CD44~-胃癌细胞,并通过无血清悬浮培养法比较两群细胞的球形体形成能力。结果免疫荧光显示,CD44蛋白在球形体中高表达;流式分析发现,贴壁的原代胃癌细胞中CD44~+细胞的比例为(30.73±7.6)%,而球形体中CD44~+细胞的比例高达(56.4±5.58)%,差异有统计学意义,P=0.009。球形体形成实验结果表明,与CD44~-细胞相比,CD44~+胃癌细胞具有更强的球形体形成能力,1 000个CD44~+及CD44~-胃癌细胞分别形成(14.33±2.52)和(1.67±0.58)个球形体,差异有统计学意义,P=0.001。通过比较发现,球形体培养对胃癌干细胞的富集效率为0.3%,而CD44对胃癌干细胞的富集效率为1.4%。结论 CD44是胃癌干细胞的表面标记蛋白,其可能成为胃癌靶向治疗的可靠靶标。
[Abstract]:Objective Cancer stem cell stem cells play an important role in the occurrence and development of malignant tumor. In this study, we investigated the important role of CSCs surface marker protein CD44 in the isolation and identification of gastric cancer stem cells. Methods 2015-11-06-2015-. 12-01 gastric cancer cells were obtained by collagenase digestion from 3 patients with gastric cancer in Tongji Hospital affiliated to Tongji Medical College of Huazhong University of Science and Technology. Some of the cells were adhered to the wall and cultured in suspension. The expression of CD44 in the spherical body of gastric cancer was detected by immunofluorescence and flow cytometry. CD44 ~ and CD44 ~-gastric cancer cells were isolated by flow cytometry. The ability of spheroid formation of the two groups of cells was compared by serum-free suspension culture. Results Immunofluorescence showed that CD44 protein was highly expressed in the spheroidium. The percentage of CD44 ~ cells in primary gastric cancer cells was 30.73 卤7.6%. The percentage of CD44~ cells in the globular body was 56.4 卤5.58. The difference was statistically significant. Compared with CD44- cells, CD44- cells have stronger spheroidogenesis ability. 1 000 CD44 ~ and CD44 ~-gastric cancer cells formed 14. 33 卤2. 52) and 1. 67 卤0. 58) spheroids, respectively. The difference was statistically significant. P0. 001. by comparison, it was found that the enrichment rate of gastric cancer stem cells in spheroid culture was 0.3%. Conclusion CD44 is the surface marker protein of gastric cancer stem cells, and it may be a reliable target for gastric cancer targeted therapy.
【作者单位】：华中科技大学同济医学院附属同济医院肿瘤中心;
【基金】：国家自然科学基金(81201820)
【分类号】：R735.2
【正文快照】： 中华肿瘤防治杂志,2017,24(3):173-177Chin J Cancer Prev Treat,2017,24(3):173-177自从20世纪末肿瘤干细胞(cancer stem cells,CSCs)理论被提出后,其在很大程度上改变了恶性肿瘤的临床诊断思维和治疗方法。该理论认为CSCs是肿瘤组织中存在的一小群具有干细胞的自我更新和分

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