MMP-2、MMP-9在JAK2 V617F阳性的骨髓增殖性肿瘤中表达增强

发布时间：2018-02-02 14:40

本文关键词： 骨髓增殖性肿瘤 JAK VF突变金属基质蛋白酶、金属基质蛋白酶　出处：《细胞与分子免疫学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的探讨基质金属蛋白酶2(MMP-2)、MMP-9在Janus激酶2 V617F(JAK2 V617F)阳性骨髓增殖性肿瘤(MPN)中的表达及意义。方法选取58例JAK2 V617F突变阳性的MPN患者,女24例及男34例,中位年龄57岁。其中包括原发性骨髓纤维化21例、血小板增多症19例、真性红细胞增多症18例。所有58例患者中包括初治组40例(再分为肝或脾肿大组28例及无肝或脾肿大组12例),治疗组18例。对照组为15例健康志愿者。应用免疫组织化学染色法检测各组骨髓组织中磷酸化JAK2(p-JAK2)、MMP-2、MMP-9的表达水平。实时荧光定量PCR方法检测JAK2 V617F与JAK2比值,计算突变量。流式细胞术检测骨髓CD34~+细胞计数。Transwell~(TM)小室检测细胞迁移力。结果 JAK2 V617F/JAK2突变量在初治组高于治疗组,p-JAK2、MMP-2、MMP-9蛋白阳性率在初治组均显著高于治疗组和对照组。Spearman相关分析显示在初治组、治疗组JAK2V617F突变量分别与MMP-2、MMP-9蛋白阳性率正相关。0.5万U/L干扰素α2b(IFN-α2b)能够抑制MPN原代细胞迁移。肝脾肿大组患者JAK2 V617F/JAK2、MMP-2、MMP-9、CD34~+细胞表达水平明显高于无肝脾肿大组。结论 MMP-2、MMP-9在JAK2 V617F阳性MPN中的表达异常升高,并与JAK2 V617F突变量密切相关。
[Abstract]:Objective to study matrix metalloproteinase 2 (MMP-2). MMP-9 in bone marrow proliferative neoplasms positive for Janus kinase 2 V617FG JAK2 V617F). Methods 58 patients with JAK2 V617F mutation positive MPN were selected. The median age was 57 years old, including 21 cases of primary myelofibrosis and 19 cases of thrombocytopenia. There were 18 cases of polycythemia vera, including 40 cases in the first treatment group (28 cases in the liver or splenomegaly group and 12 cases in the non-hepatomegaly group). 18 patients in the treatment group and 15 healthy volunteers in the control group were used to detect the phosphorylated JAK2 p-JAK2 + MMP-2 in bone marrow tissue of each group by immunohistochemical staining. The expression level of MMP-9. The ratio of JAK2 V617F to JAK2 was detected by real-time fluorescence quantitative PCR. Calculation of mutations. Detection of bone marrow CD34 ~ cell count by flow cytometry. Results the mutation amount of JAK2 V617F / JAK2 was higher in the initial treatment group than in the treatment group. The positive rate of p-JAK2MP-2MMP 9 protein in the primary treatment group was significantly higher than that in the treatment group and the control group. The amount of JAK2V617F mutation in the treatment group was different from that of MMP-2. Positive correlation of MMP-9 protein. 5,000 U / L interferon 伪 2bn- 伪 2b). In hepatosplenomegaly group, JAK2 V617F / JAK2 was inhibited. The expression level of MMP-9 ~ + CD34 ~ cells was significantly higher than that of non-hepatosplenomegaly group. Conclusion MMP-2. The expression of MMP-9 was increased in JAK2 V617F positive MPN, and was closely related to the JAK2 V617F mutation.
【作者单位】：保定市第一医院;承德医学院;河北大学附属医院;
【基金】：2016年河北省科技计划项目(162777120D)
【分类号】：R733.3
【正文快照】： 骨髓增殖性肿瘤(myeloproliferative neoplasms,MPN),是一类起源于造血干细胞,以骨髓中一系或多系血细胞异常增殖为特点的血液系统克隆性疾病。研究报道,在绝大多数的真性红细胞增多症(polycythemia vera,PV)及半数的原发性血小板增多症(essential thrombocythema,ET)、原发性

【参考文献】