miR-30d在舌鳞状细胞癌组织中表达及反义miR-30d对细胞增殖、侵袭能力的影响

发布时间：2018-02-04 08:52

本文关键词： 舌鳞状细胞癌 miR-d 反义寡核酸技术细胞增殖细胞侵袭　出处：《口腔医学研究》2017年12期 　论文类型：期刊论文

【摘要】：目的:探讨miR-30d在舌鳞状细胞癌组织中表达及反义miR-30d对细胞增殖、侵袭能力的影响。方法:选取2013年3月~2015年4月在我院行根治性手术治疗的TSCC患者68例,实时荧光定量PCR检测TSCC组织和癌旁正常组织中miR-30d基因表达,培养舌癌SCC-4细胞,分为ASO-miR-30d组、ASO-阴性对照组和空白对照组,检测各组细胞中miR-30d基因表达,MTT法检测各组细胞增殖能力,Transwell法检测各组细胞迁移和侵袭能力。结果:TSCC组织中miR-30d相对表达量(1.89±0.21),显著高于癌旁正常组织的(1.31±0.16),差异有统计学意义(P=0.000);TSCC组织中miR-30d相对表达量与TNM分期、分化程度和颈淋巴结转移有关(P0.05);与空白对照组和ASO-阴性对照组比较,ASO-miR-30d组细胞中miR-30d相对表达量降低,差异有统计学意义(P0.05);MTT实验结果显示,与空白对照组和ASO-阴性对照组比较,ASO-miR-30d组24h、48h、72h和96h时细胞A值均降低,差异均有统计学意义(P0.05);与空白对照组和ASO-阴性对照组比较,ASO-miR-30d组迁移细胞数和侵袭细胞数均减少,差异均有统计学意义(P0.05)。结论:miR-30d在TSCC组织中呈高表达,反义miR-30d可有效减少TSCC细胞增殖,抑制细胞迁移和侵袭能力。
[Abstract]:Objective: to investigate the expression of miR-30d in tongue squamous cell carcinoma and the effect of antisense miR-30d on cell proliferation. Methods: from March 2013 to April 2015, 68 patients with TSCC underwent radical operation in our hospital. Real-time fluorescence quantitative PCR was used to detect the expression of miR-30d gene in TSCC tissues and adjacent normal tissues. SCC-4 cells of tongue cancer were cultured and divided into ASO-miR-30d group. ASO- negative control group and blank control group were used to detect the expression of miR-30d gene in the cells of each group. Transwell assay was used to detect the ability of cell migration and invasion. Results the relative expression of miR-30d in TSCC was 1.89 卤0.21). It was significantly higher than that in normal tissues adjacent to cancer (1.31 卤0.16), and the difference was statistically significant (P < 0.05). The relative expression of miR-30d in TSCC was related to TNM stage, differentiation and cervical lymph node metastasis (P0.05). Compared with blank control group and ASO- negative control group, the relative expression of miR-30d in ASO-miR-30d group was significantly lower than that in ASO-miR-30d group (P 0.05). The results of MTT test showed that compared with the blank control group and ASO- negative control group, the A value of ASO-miR-30d group decreased at 24 h, 48 h, 72 h and 96 h, respectively. The difference was statistically significant (P 0.05). Compared with blank control group and ASO- negative control group, the number of migration cells and the number of invasive cells in ASO-miR-30d group were lower than those in ASO-miR-30d group. The difference was statistically significant (P 0.05). Conclusion the expression of TSCC cells is high at 30 d, and antisense miR-30d can effectively reduce the proliferation of TSCC cells. Inhibition of cell migration and invasion.
【作者单位】：广州市中西医结合医院口腔科;中山大学第一附属医院口腔科;
【基金】：广东省自然科学基金(编号:2014A030310076)
【分类号】：R739.86
【正文快照】： 0.05).Compared with the blank control group and the ASO-negative control group,the number of migrating cells andthe number of invasive cells in ASO-miR-30dgroup weredecreased(P0.05).Conclusion:miR-30dwas highly ex-pressed in TSCC tissues.Antisense miR-3

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