血清外泌体miR-21在肺癌中的表达水平及其诊断效能

发布时间：2018-02-13 20:56

本文关键词： 外泌体血清肺癌诊断标志物 miR-　出处：《实用医学杂志》2017年16期 　论文类型：期刊论文

【摘要】：目的探索外泌体miR-21在肺癌患者血清中的表达水平及诊断效能。方法收集南方医院肺癌患者94例,肺结核患者29例,健康对照26例。纯化鉴定血清外泌体,提取miRNA,进行RT-PCR,比较各组间miR-21表达水平。利用ROC曲线评价外泌体miR-21诊断效能。结果纯化样品具备已有报道的外泌体的典型特征。外泌体miR-21表达水平在肺癌、肺结核、健康对照3组间存在显著性差异;外泌体miR-21在肺癌组中显著升高,肺结核组显著降低。肺癌组中,早、晚期肺癌患者外泌体miR-21与健康对照间均存在显著性差异。外泌体miR-21的ROC曲线的曲线下面积为0.702,且具有较好的特异性。结论血清外泌体miR-21具有较高的诊断效能,有望作为诊断标志物,辅助肺癌临床诊断。
[Abstract]:Objective to investigate the expression level and diagnostic efficacy of exocrine miR-21 in serum of lung cancer patients. Methods 94 patients with lung cancer, 29 patients with pulmonary tuberculosis and 26 healthy controls were collected. MiRNAs were extracted, and the expression level of miR-21 was compared among the groups. The diagnostic efficacy of miR-21 was evaluated by ROC curve. Results the purified samples had the typical characteristics of exocrine bodies. The miR-21 expression levels of exosomes were found in lung cancer, pulmonary tuberculosis, pulmonary tuberculosis, lung cancer, pulmonary tuberculosis, pulmonary tuberculosis, lung cancer, pulmonary tuberculosis, pulmonary tuberculosis, pulmonary tuberculosis and lung cancer. The exocrine miR-21 in lung cancer group was significantly higher than that in pulmonary tuberculosis group, while in lung cancer group, it was early in lung cancer group. There was significant difference between exocrine miR-21 and healthy control in patients with advanced lung cancer. The area of ROC curve of exocrine miR-21 was 0.702, and it had good specificity. Conclusion Serum exocrine miR-21 has higher diagnostic efficacy. It is expected to be used as a diagnostic marker to assist the clinical diagnosis of lung cancer.
【作者单位】：南方医科大学南方医院临床医学实验研究中心;南方医科大学南方医院检验科;
【基金】：广东省自然科学基金(编号:2015A030313293) 广东省科技计划项目(编号:2013B021800314) 广州市科技计划项目(编号:1563000220)
【分类号】：R730.43;R734.2

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