血浆长链非编码RNA HIT对乳腺癌的诊断价值

发布时间：2018-02-16 00:32

  本文关键词： 乳腺癌 长链非编码RNA HIT 生物标志物　出处：《广东医学》2017年08期 　论文类型：期刊论文

【摘要】：目的探讨转化生长因子-β(TGF-β)诱导的HOXA转录物(HOXA transcript induced by TGF-β,HIT)在乳腺癌女性血浆中的表达情况及其对乳腺癌的潜在诊断价值。方法收集乳腺癌女性患者血浆标本124例(乳腺癌组)、乳腺良性疾病患者血浆标本59例(乳腺良性疾病组)及健康体检女性血浆标本48例(对照组),采用实时定量逆转录PCR(real time RT-PCR)方法分别检测各组标本中HIT的表达水平,分析HIT与乳腺癌临床病理特征的相关性,绘制受试者工作特征曲线(ROC)以评估血浆HIT对乳腺癌的诊断效能。结果 HIT在乳腺癌组血浆中表达水平[0.12(0.02,0.33)]明显低于对照组[0.55(0.20,1.59)],差异有统计学意义(U=1 020,P0.000 1),HIT在乳腺良性疾病组血浆中表达水平[0.10(0.02,0.19)]明显低于对照组,差异有统计学意义(U=308.5,P0.000 1),HIT在乳腺癌组血浆中表达水平低于乳腺良性疾病组,但差异无统计学意义(U=3 028,P=0.060 2)。血浆中HIT的表达水平与雌激素受体(ER)(P=0.021)和淋巴结转移(P=0.033)明显相关。血浆HIT诊断乳腺癌的ROC曲线下面积(AUC)为0.799,敏感度和特异度分别为83.3%和66.7%。结论血浆中低表达的lncRNA HIT可能成为乳腺癌诊断的一个潜在的新型生物标志物。
[Abstract]:Objective to investigate the expression of HOXA transcript induced by TGF- 尾 in the plasma of women with breast cancer and its potential diagnostic value. Methods the plasma samples of 124 women with breast cancer were collected. The expression of HIT was detected by real-time quantitative reverse transcription PCR(real time RT-PCR method in 59 plasma samples from patients with adenocarcinoma, 59 patients with benign breast disease (benign breast disease) and 48 healthy women (control group). To analyze the correlation between HIT and clinicopathological features of breast cancer. Results the level of plasma HIT expression in breast cancer group [0.12 0. 02 卤0. 33] was significantly lower than that in control group [0. 55 0. 20 卤1. 59], and the difference was statistically significant. The expression level of plasma in the disease group was significantly lower than that in the control group. The difference was statistically significant (P < 0.05). The plasma level of Hit in breast cancer group was lower than that in benign breast disease group. However, there was no significant difference between the two groups. The expression of HIT in plasma was significantly correlated with that of estrogen receptor ERP (0.021) and lymph node metastasis (P < 0.033). The area under the ROC curve of plasma HIT for diagnosis of breast cancer was 0.799, the sensitivity and specificity were 83.3% and 83.3%, respectively. Conclusion the low expression of lncRNA HIT in plasma may be a potential new biomarker for diagnosis of breast cancer.
【作者单位】： 西南医科大学附属医院检验科;四川省肿瘤医院检验科;
【基金】：四川省科学技术厅专项资金项目(编号:14JC0801)
【分类号】：R737.9;R730.43
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