EphA2与VEGF-C在胃癌中的表达及其与淋巴管生成的关系

发布时间：2018-02-22 00:02

本文关键词： 胃肿瘤受体 Eph家族血管内皮生长因子C 淋巴管生成　出处：《中国普通外科杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的:探讨胃癌组织中EphA2与VEGF-C的表达及临床意义。方法:用免疫组化法检测82例胃癌组织及其癌旁组织中EphA2与VEGF-C蛋白的表达,以及淋巴管密度(LVD);用real-timePCR检测20例胃癌组织及其癌旁组织中EphA2与VEGF-Cm RNA的表达。分析EphA2与VEGF-C表达与患者临床病理特点及淋巴管生成的关系。结果:EphA2与VEGF-C在胃癌组织中的阳性表达率均明显高于癌旁正常组织(65.8%vs.42.6%;71.9%vs.39.0%,均P0.05)。EphA2与VEGF-C的高表达与肿瘤浸润深度、淋巴结转移、TNM分期有关(均P0.001)。EphA2蛋白与VEGF-C蛋白高表达的胃癌组织中LVD计数分别明显高于两者低表达胃癌组织(15.25±5.41vs.10.95±5.41,P=0.001;14.87±5.71vs.11.00±5.01,P=0.006)。胃癌组织中EphA2与VEGF-C蛋白以及m RNA表达均呈正相关(r=0.375,P=0.001;r=0.559,P=0.01)。结论:EphA2与VEGF-C在胃癌组织中均呈高表达,且可能共同促进淋巴管生成与胃癌淋巴结转移。
[Abstract]:Objective: to investigate the expression and clinical significance of EphA2 and VEGF-C in gastric carcinoma. Methods: the expression of EphA2 and VEGF-C in 82 cases of gastric cancer and its adjacent tissues were detected by immunohistochemical method. Real-timePCR was used to detect the expression of EphA2 and VEGF-Cm RNA in 20 cases of gastric carcinoma and its adjacent tissues. The relationship between the expression of EphA2 and VEGF-C and clinicopathological features and lymphangiogenesis was analyzed. The positive expression rates of EphA2 and VEGF-C were significantly higher than those of normal tissues (65.8vs.42.6) and 71.9vs.39.0.The expression of EphA2 and VEGF-C and the depth of tumor invasion were all significantly higher than those in the adjacent normal tissues. The number of LVD in gastric cancer tissues with high expression of P0.001, EphA2 protein and VEGF-C protein was significantly higher than that in low expression gastric cancer tissues (15.25 卤5.41vs.10.95 卤5.41vs.0.001 卤5.71vs.11.00 卤5.01vs.11.00 卤5.01P0.006.There was a positive correlation between the expression of EphA2 and VEGF-C protein and the expression of m RNA in gastric cancer tissues. On the high expression of VEGF-C and EphA2 in gastric carcinoma, And it may promote lymphangiogenesis and lymph node metastasis of gastric cancer.
【作者单位】：湖南省长沙市中心医院普通外科;中南大学湘雅医院普通外科;
【基金】：高等学校博士学科点专项科研基金资助项目(20130162110026)
【分类号】：R735.2

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