大鼠腮腺放射性损伤早期SSB1的表达变化及细胞凋亡研究

发布时间：2018-02-28 11:29

本文关键词： SSB1 腮腺放射性损伤 DNA双链断裂修复细胞凋亡　出处：《广西医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:研究不同放射剂量腮腺组织及同一剂量放射后不同时间点腮腺组织SSB1的动态表达及细胞凋亡规律,为涎腺放射性损伤修复机制的研究提供理论依据。方法:将大鼠随机分为对照组,不同剂量放射6组,同一剂量放射后不同时间点8组,建立大鼠放射模型并且取材;应用TUNEL方法检测正常组,不同剂量放射组及同一剂量放射后不同时间点的细胞凋亡情况;应用IHC方法检测正常组,不同剂量放射组及同一剂量放射后不同时间点SSB1蛋白在腮腺组织中分布及表达情况;应用RT-qPCR方法检测正常组,不同剂量放射组及同一剂量放射后不同时间点SSB1mRNA在腮腺组织中表达规律;应用WB方法检测正常组,不同剂量放射组及同一剂量放射后不同时间点SSB1蛋白在腮腺组织中表达情况。结果:TUNEL结果示不同剂量放射后,凋亡细胞阳性率随放射剂量先增高后降低,并在12Gy组凋亡细胞阳性率达到最高(P0.05)。同一剂量放射后不同时间点组,随着时间的变化,1小时组凋亡细胞阳性率最高(P0.05),后期降低且无明显变化;IHC,RT-q PCR及WB结果示经不同剂量照射和同一剂量照射后,SSB1表达明显增加。不同剂量放射后,SSB1的表达随剂量先增高后降低,并在12Gy组表达量达到最高(P0.05)。同一剂量辐射后,SSB1的表达随放射后时间呈先增长后减少的趋向,前期相对表达量高于在后期(P0.05)。结论:正常腮腺组织中也能表达一定量的SSB1;辐射能诱导大鼠腮腺组织SSB1的表达增加;SSB1的表达与辐射剂量有关,与辐射后时间长短也有一定的关系;细胞凋亡是大鼠腮腺放射早期的损伤形式,其与SSB1的表达规律大体上具有平行性,呈现先增高后降低的趋向。这些成果均为进一步研究涎腺放射性损伤修复机制提供新的理论依据。
[Abstract]:Objective: To study the dynamic expression and apoptosis of the parotid gland tissue at different time points SSB1 of different radiation dose of parotid gland and the same dose of radiation, and provide a theoretical basis for study of the mechanism of radiation damage repair of salivary gland. Methods: the rats were randomly divided into control group, different doses of radiation 6 groups, the same after different doses of radiation the 8 time points of group, to establish a rat model of radiation and were detected by TUNEL method; the normal group, the cell apoptosis at different time points in different doses of radiation group and the same dose of radiation was detected by IHC method; the normal group, the radiation group and the same dose at different time points after SSB1 protein in parotid gland the distribution and expression was detected by RT-qPCR method; the normal group, different doses of radiation group and the same dose at different time points after SSB1mRNA expression in parotid tissues was detected by WB method; The normal group, the expression of SSB1 protein in different time points of parotid gland in different doses of radiation group and the same dose after radiotherapy. Results: TUNEL results showed that different doses of radiation, the positive rate of apoptotic cells with radiation dose increased firstly and then decreased, and the positive rate of apoptotic cells in 12Gy group reached the highest (P0.05) the same. The radiation dose at different time points after group, with the change of time, 1 hours of apoptotic cells group had the highest positive rate (P0.05), later reduced and no obvious change; IHC, RT-q PCR and WB results are shown with different doses of irradiation and the same dose after irradiation, the expression of SSB1 was increased significantly. After different doses of radiation, SSB1 the expression first increased with the dose and then decreased, and the highest expression in the 12Gy group (P0.05). The same dose of radiation, the expression of SSB1 in a time trend with radiation was reduced after the first growth, the relative expression is higher than that in the later (P0.05). Conclusion: the normal parotid gland The organization also can express a certain amount of SSB1; increase the expression of radiation can induce SSB1 parotid gland tissue of rats; the expression of SSB1 was correlated with the dose and time after radiation in some degree; apoptosis is an early form of radiation injury of rat parotid gland, and the expression of SSB1 in parallel with the law the trend of first increased and then decreased. These results provide new evidence for further study on the mechanism of radiation damage repair of salivary gland.

【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.91;R730.55

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