黄连碱经活性氧中介物-线粒体途径诱导肺癌NCI-H1650细胞凋亡

发布时间：2018-03-01 21:26

本文关键词： 黄连碱细胞凋亡活性氧中介物非小细胞肺癌 NCI-H细胞　出处：《实用医学杂志》2017年24期 　论文类型：期刊论文

【摘要】：目的研究黄连碱对人非小细胞肺癌NCI-H1650细胞增殖的影响和其用于人非小细胞肺癌临床治疗的潜在价值。方法 MTT法分析黄连碱处理对NCI-H1650细胞增殖的影响,Western blot检测细胞凋亡相关基因Bax、Bcl-2和细胞色素C的蛋白表达水平。流式细胞术分析Annexin V/PI标记的细胞凋亡。荧光酶标仪分析细胞内活性氧中介物水平。结果黄连碱能够以时间和剂量依赖性的方式显著抑制NCIH1650细胞增殖。黄连碱处理24 h诱导NCI-H1650细胞内活性氧中介物水平增加和细胞凋亡,同时细胞内Bax基因表达上调、Bcl-2基因表达下调,并发生细胞色素C从线粒体向细胞浆的转移。流式细胞术检测发现黄连碱处理导致NCI-H1650细胞凋亡发生。使用活性氧抑制物N-乙酰半胱氨酸则可显著抵抗黄连碱对NCI-H1650细胞的增殖抑制和细胞凋亡。结论黄连碱通过诱导活性氧中介物水平增加激发NCI-H1650细胞发生线粒体途径介导的细胞凋亡。黄连碱具有用于抗人非小细胞肺癌临床治疗的潜在价值。
[Abstract]:Objective to study the effect of Coptidine on proliferation of human non-small cell lung cancer (NSCLC) NCI-H1650 cells and its potential value in clinical treatment of human NSCLC. Methods MTT assay was used to analyze the effect of Coptis alkaloids on the proliferation of NCI-H1650 cells. The protein expression level of apoptosis related gene BaxanBcl-2 and cytochrome C. flow cytometry was used to analyze the apoptosis of Annexin V / Pi labeled cells. Fluorescence enzyme labeling instrument was used to analyze the level of reactive oxygen species in cells. Results Coptis alkaloids could be used as time and agent. In a dose-dependent manner, the proliferation of NCIH1650 cells was significantly inhibited, and the level of reactive oxygen species (Ros) mediators and apoptosis in NCI-H1650 cells were increased after treatment with Coptis for 24 h. At the same time, the expression of Bax gene was up-regulated and the expression of Bcl-2 gene was down-regulated. Flow cytometry showed that the apoptosis of NCI-H1650 cells was induced by the treatment of Coptis alkaloids. N- acetylcysteine, a reactive oxygen species inhibitor, could significantly resist the effect of Coptis alkaloids on NCI-H1650. Conclusion Coptis alkaloids can induce mitochondrial pathway apoptosis in NCI-H1650 cells by increasing the level of reactive oxygen species mediators. Coptis alkaloids have potential value in the clinical treatment of human non-small cell lung cancer.
【作者单位】：湖北省宜昌市第二人民医院放化疗一科;湖北省宜昌市第二人民医院检验科;
【基金】：宜昌市科技局公益类科技项目基金资助(编号:A16-301-29)
【分类号】：R734.2

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