毛蕊异黄酮增强顺铂对胃癌细胞增殖的抑制作用

发布时间：2018-03-02 03:23

本文关键词： 毛蕊异黄酮顺铂 BGC 胃癌增殖　出处：《中国医科大学学报》2017年01期 　论文类型：期刊论文

【摘要】：目的观察毛蕊异黄酮对顺铂抑制胃癌细胞增殖的增强作用。方法将BGC823细胞分为4组,分别用生理盐水、顺铂、毛蕊异黄酮和2种药物联合处理,检测不同时间点、不同处理因素下的细胞增殖情况。进一步检测影响细胞增殖的相关蛋白,确定毛蕊异黄酮联合顺铂抑制胃癌细胞增殖的方式。结果对细胞作用24 h后,20μg/m L顺铂,10μg/m L毛蕊异黄酮,以及2种药物联合处理组对BGC823细胞的增殖抑制率分别为56.44%±2.08%,9.52%±2.77%和74.44%±0.82%。药物联合使用后对细胞的抑制率明显高于单个药物处理组,各组数据间差异有统计学意义(P0.05)。并且无论是在蛋白水平还是RNA水平上,毛蕊异黄酮均可以显著增强顺铂对Cyclin D1,CDK4和CDK6蛋白的抑制作用。结论毛蕊异黄酮可以显著提高顺铂对BGC823细胞增殖的抑制率,2种药物联合使用可以降低顺铂单独使用所导致的毒副作用。
[Abstract]:Objective to observe the effect of Cisoflavone on the proliferation of gastric cancer cells. Methods BGC823 cells were divided into 4 groups, which were treated with normal saline, cisplatin, isoflavone and two drugs respectively, and detected at different time points. Cell proliferation under different treatment factors. Further detection of cell proliferation related proteins, to determine the way to inhibit the proliferation of gastric cancer cells. Results after 24 hours of cell contact, 20 渭 g / mL cisplatin 10 渭 g / m L Cisoflavone, The inhibition rates of proliferation of BGC823 cells were 56.44% 卤2.089.52% 卤2.77% and 74.44% 卤0.82% respectively in the combined treatment group, and the inhibition rate of the two drugs was significantly higher than that of the single drug treatment group. There was significant difference in the data of each group (P 0.05). And whether in protein level or RNA level, The inhibitory effect of Cisplatin on Cyclin D1 CDK4 and CDK6 protein was significantly enhanced. Conclusion Cisoflavone can significantly increase the inhibition rate of Cisplatin on the proliferation of BGC823 cells. The combination of two drugs can reduce the use of cisplatin alone. The toxic side effects caused.
【作者单位】：锦州医科大学附属第三医院肿瘤科;
【分类号】：R735.2

【参考文献】