PTEN和NDRG1在结直肠腺癌中的表达及其与预后的关系

发布时间：2018-03-03 00:30

本文选题：PTEN蛋白　切入点：NDRG1蛋白　出处：《昆明医科大学》2015年硕士论文　论文类型：学位论文

【摘要】：[目的]PTEN、NDRG1是肿瘤发生发展过程中的重要因子。本文探讨PTEN、 NDRG1在结直肠腺癌的表达特点及其与结直肠腺癌患者预后的关系。[方法]采用免疫组化方法对PTEN、NDRG1蛋白在21例正常结直肠粘膜、30例结直肠腺瘤、91例结直肠腺癌样本中的表达情况进行检测,还用计算机图像分析系统对免疫组化结果进行定量分析。并对有完整随访资料的54例结直肠腺癌患者的数据进行统计以期了解两个因子对患者预后的影响。[结果]1. PTEN蛋白在正常结直肠粘膜、结直肠腺瘤、结直肠腺癌中的表达阳性率分别是90.48%、70.00%、54.95%,其蛋白表达呈逐渐降低趋势；PTEN蛋白表达与结直肠腺癌淋巴结转移呈负相关关系(0.01P0.05,rs’=-0.240)；与结直肠腺癌分化程度和患者的年龄、性别之间差异无关(P0.05)。2. NDRG1蛋白在正常结直肠粘膜、结直肠腺瘤、结直肠腺癌的表达阳性率分别是57.14%、63.33%、76.92%,其蛋白表达逐渐增高趋势；NDRG1蛋白表达与结直肠腺癌淋巴结转移呈正相关关系(0.01P0.05,rs’=0.218)；与结直肠腺癌分化程度和患者性别之间差异无关(P0.05)但与病人的年龄差异有关(P0.05)。3.在结直肠腺癌中,PTEN及NDRG1蛋白的表达呈负相关关系(P0.05,rs’=-0.251)。4.PTEN蛋白表达情况是影响结直肠腺癌患者预后的因素。5.流行病学诊断实验评价方法显示：联合检测PTEN和NDRG1敏感度为74.07%,特异度为52.38%,准确度为70.54%,阳性预测值为88.89%,阴性预测值为28.21%。[结论]1. PTEN失表达有可能成为结直肠腺癌癌变早期的分子生物学标记物之一,并可作为预测结直肠腺癌患者预后的指标之一。2. NDRG1与结直肠腺癌的发生发展有关,但不能作为临床预测结直肠腺癌患者预后的指标。3.联合检测PTEN和NDRG1,可作为疑难病例的辅助诊断指标。
[Abstract]:[objective] to investigate the expression of PTENN NDRG1 in colorectal adenocarcinoma and its relationship with the prognosis of colorectal adenocarcinoma. [methods] Immunohistochemical method was used to study the expression of PTENN NDRG1 protein in 21 patients with colorectal adenocarcinoma. The expression of adenoma and adenoma was detected in 91 cases of colorectal adenoma, 30 cases of normal colorectal mucosa and 30 cases of colorectal adenoma. Computer image analysis system was also used to quantitatively analyze the immunohistochemical results. The data of 54 colorectal adenocarcinoma patients with complete follow-up data were analyzed in order to understand the influence of two factors on the prognosis of the patients. PTEN protein in normal colorectal mucosa, The positive rates of PTEN protein expression in colorectal adenoma and colorectal adenocarcinoma were 90.48 and 70.00and 54.95, respectively. The expression of PTEN protein decreased gradually and was negatively correlated with lymph node metastasis of colorectal adenocarcinoma. NDRG1 protein in normal colorectal mucosa, colorectal adenoma, The positive expression rates of colorectal adenocarcinoma were 57.14 and 63.33, respectively. The expression of NDRG1 protein increased gradually. The expression of NDRG1 protein was positively correlated with lymph node metastasis of colorectal adenocarcinoma. There was no correlation between NDRG1 protein expression and lymph node metastasis of colorectal adenocarcinoma, and there was no correlation between NDRG1 protein expression and differentiation degree of colorectal adenocarcinoma and sex difference between patients. In colorectal adenocarcinoma, there was a negative correlation between the expression of PTEN and NDRG1 protein. P0.05 rstium-0.251. 4. PTEN protein expression was a factor influencing the prognosis of colorectal adenocarcinoma patients .5.The method of epidemiological diagnostic evaluation showed that PTEN protein expression in colorectal adenocarcinoma patients was negative correlation with that of P0.05 P0.05 protein expression in colorectal adenocarcinoma patients. The epidemiological diagnostic evaluation method showed that the expression of PTEN protein was negatively related to the prognosis of colorectal adenocarcinoma patients. The results showed that the sensitivity of combined detection of PTEN and NDRG1 was 74.07, the specificity was 52.38, the accuracy was 70.54, the positive predictive value was 88.89, and the negative predictive value was 28.21. [conclusion] 1.The loss of PTEN expression may become one of the molecular biomarkers in the early stage of colorectal adenocarcinoma carcinogenesis. NDRG1 may be one of the predictors of the prognosis of colorectal adenocarcinoma. NDRG1 is related to the occurrence and development of colorectal adenocarcinoma. But it can not be used as a clinical predictor of prognosis of colorectal adenocarcinoma. Combined detection of PTEN and NDRG1 can be used as an auxiliary diagnostic index in difficult cases.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.34

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