溶瘤腺病毒ATV联合顺铂协同抑制A 549细胞的研究

发布时间：2018-03-03 13:35

本文选题：溶瘤腺病毒　切入点：顺铂　出处：《中国病原生物学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的探讨双特异性溶瘤腺病毒ATV与化疗药物顺铂联用对人肺癌细胞A 549治疗的增效减毒作用。方法将构建的溶瘤腺病毒ATV感染A 549细胞后进行Annexin V检测,分析ATV对肿瘤细胞的抑制方式;单用ATV、顺铂以及两者联合作用于人肺癌A 549细胞,采用MTS法检测细胞体外增殖情况,采用划痕试验、Transwell迁移试验和Transwell侵袭试验检测细胞的迁移侵袭情况;构建A 549皮下移植瘤裸鼠模型,单独注射重组腺病毒、顺铂以及重组腺病毒和顺铂联合使用,观察监测肿瘤生长情况,评价溶瘤腺病毒联合顺铂对肿瘤的体内抑制作用。结果双特异性重组腺病毒ATV主要通过诱导A 549细胞凋亡达到对细胞的杀伤;ATV联合顺铂体外杀伤A 549细胞具有一定的剂效和时效关系,且其抑制作用显著强于ATV及顺铂单独使用;ATV联合顺铂作用下,可使A 549细胞迁移侵袭能力降低,且能显著抑制小鼠皮下移植瘤生长,延长模型小鼠生存期。结论 ATV可诱导肿瘤细胞凋亡,ATV与化疗药物顺铂联用可增强对A549细胞生长及迁移侵袭能力的抑制作用,即具有抑制肺癌细胞A 549的增效减毒作用。
[Abstract]:Objective to investigate the synergistic and attenuated effects of bispecific adenovirus (ATV) combined with cisplatin on human lung cancer cell line A549. Methods Annexin V was detected after A549 cells were infected with adenovirus ATV. The inhibition of ATV on tumor cells was analyzed, and the proliferation of human lung cancer A549 cells was detected by MTS assay, which was treated with ATV, cisplatin and the combination of ATV, cisplatin and both. The cell migration and invasion were detected by scratch test and Transwell invasion test, the nude mice model of A549 subcutaneous transplanted tumor was established, and the recombinant adenovirus, cisplatin, recombinant adenovirus and cisplatin were injected separately. Observe and monitor tumor growth, Results the recombinant adenovirus ATV can kill A549 cells by inducing A549 cell apoptosis and killing A549 cells in vitro. The relationship between the effect of the agent and the aging, Its inhibitory effect was stronger than that of ATV and cisplatin alone combined with cisplatin, which decreased the migration and invasion ability of A549 cells and significantly inhibited the growth of subcutaneous transplanted tumor in mice. Conclusion the combination of ATV and cisplatin can inhibit the growth and migration of A549 cells, which is a synergistic and detoxifying effect of inhibiting lung cancer cell line A549.
【作者单位】：长春中医药大学药学院;军事医学科学院军事兽医研究所病毒研究室;长春中医药大学基础医学院;延边大学医学院;
【基金】：国家新药创制科技重大专项(No.2014ZX09304314-002) 吉林省产业技术创新战略联盟项目(No.20140309006YY) 吉林省重大科技攻关项目(No.20150201002YY) 长春市重大科技攻关项目(No.16ss11)
【分类号】：R734.2

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