乳腺癌中RNF2的表达及其临床意义

发布时间：2018-03-05 10:12

本文选题：乳腺肿瘤　切入点：环指蛋白　出处：《临床与实验病理学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的观察RNF2在乳腺病变组织及乳腺上皮细胞系中的表达,探讨其表达与乳腺癌临床病理特征的关系。方法运用免疫组化En Vision两步法检测21例乳腺良性病变组织和112例乳腺癌组织中RNF2蛋白的表达,分析其表达与乳腺癌临床病理特征的关系。应用qRT-PCR法检测2株乳腺正常上皮细胞系和5株乳腺癌细胞系中RNF2基因的表达。结果乳腺癌组织中RNF2蛋白表达水平与乳腺良性病变组织相比显著升高(P0.05),且其表达与乳腺癌肿瘤直径、腋窝淋巴结转移数目、TNM分期显著相关(P均0.05);与患者年龄、组织学分级、ER、PR、HER-2表达情况无显著相关(P均0.05);乳腺癌细胞系RNF2 mRNA水平亦高于乳腺良性上皮细胞系(P0.05)。结论 RNF2在乳腺癌组织中高表达,可作为乳腺癌治疗的潜在靶点。
[Abstract]:Objective To observe the expression of RNF2 in breast carcinoma tissue and mammary epithelial cell lines, to investigate the relationship between the expression and clinicopathological features of breast cancer. The expression of RNF2 protein immunohistochemistry method using En Vision two step method was used to detect 21 cases of benign breast lesions and 112 cases of breast cancer and the relationship between their expression and clinical pathological features breast cancer. The expression of qRT-PCR was used to detect 2 strains of normal breast epithelial cells and 5 strains of RNF2 gene in breast cancer cell lines. The RNF2 protein in breast carcinoma and expression of benign breast lesions were significantly higher than (P0.05), and the expression of breast cancer and tumor diameter, number of metastatic lymph nodes. TNM stage were significantly correlated (P 0.05); and the patients age, histological grade, ER, PR, HER-2 expression had no significant correlation (P 0.05); breast cancer cell line RNF2 mRNA level is also higher than that in benign breast epithelium Cell line (P0.05). Conclusion RNF2 is highly expressed in breast cancer tissue and can be used as a potential target for the treatment of breast cancer.

【作者单位】：安徽医科大学病理学教研室;安徽医科大学基础医学院生物药物研究所;
【分类号】：R737.9

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