自噬基因ARHI与LC3在宫颈癌中的表达及临床意义

发布时间：2018-03-05 10:23

本文选题：自噬　切入点：宫颈癌　出处：《青岛大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:通过分别检测并比较在正常宫颈组织、宫颈上皮内瘤变(CINII-III)组织、宫颈癌组织中自噬基因ARHI与LC3的表达水平,并分析两者与宫颈癌某些临床病理特征之间的关系以及两者之间的相关性,探讨自噬基因ARHI、LC3在宫颈癌发生、发展中的作用。方法:随机选取2014年11月至2016年10月于青岛大学附属医院崂山院区妇科初次收治100例患者的宫颈组织,20例正常宫颈组织、30例宫颈上皮内瘤变(CINII-III)组织、50例宫颈癌组织经免疫组化染色后ARHI和LC3的表达水平,并运用χ~2检验比较各组ARHI、LC3的表达水平以及两者与宫颈癌某些临床病理特征间的关系,用Spearman法分析宫颈癌中ARHI和LC3之间的相关性。结果:1.ARHI在正常宫颈组、宫颈上皮内瘤变(CINII-III)组及宫颈癌组中的阳性表达率依次为:85.0%,73.3%,54.0%,呈下降趋势,组间差异有统计学意义(χ~2=7.145,P0.05)。LC3在正常宫颈组、宫颈上皮内瘤变(CINII-III)组及宫颈癌组中的阳性表达率依次为:95.0%,56.7%,38.0%,表达下调,组间差异有统计学意义(χ~2=18.801,P0.05)。表明ARHI、LC3与宫颈癌的发生、发展密切相关。2.在宫颈癌组织中,ARHI、LC3的表达水平与临床分期及组织分化程度有关,差异有统计学意义(P0.05),而与淋巴结是否转移、年龄、SCC无明显相关性(P0.05)。3.在宫颈癌组织中,ARHI、LC3的表达具有正相关性(r=0.43,p0.05)。4.在上述100例患者中HPV感染呈阳性者共68例,根据HPV感染程度的不同分为3组,随着HPV感染程度的加重,ARHI、LC3的表达水平均呈下降趋势,但LC3在三组中的组间差异有统计学意义(χ~2=10.583,P0.05),表明LC3的表达水平与HPV感染程度密切相关,而ARHI的表达水平则与HPV感染程度无统计学意义(P0.05)。结论:ARHI、LC3表达水平的降低与缺失可能参与了宫颈癌的发生、发展过程,而且两者具有相关性,且LC3的表达水平与HPV感染程度有关。
[Abstract]:Objective: to detect and compare the expression of autophagy gene ARHI and LC3 in normal cervical tissues, cervical intraepithelial neoplasia (CINII-III) tissues and cervical cancer tissues. The relationship between the two genes and some clinicopathological features of cervical cancer was analyzed, and the relationship between them was analyzed to explore the role of autophagy gene ARHILLC3 in the occurrence of cervical cancer. Methods: from November 2014 to October 2016 in Laoshan Hospital of Qingdao University, 20 cases of normal cervical tissue and 30 cases of cervical intraepithelial neoplasia (CINII-III) were selected randomly. Expression of ARHI and LC3 in 50 cases of cervical carcinoma by immunohistochemical staining. The expression level of ARHILC3 and the relationship between ARHILC3 and some clinicopathological features of cervical cancer were compared by 蠂 ~ 2 test. The correlation between ARHI and LC3 in cervical cancer was analyzed by Spearman method. The positive expression rate of CINII-III in cervical intraepithelial neoplasia group and cervical cancer group was: 85.0% and 73.3% and 54.0% respectively, which showed a downward trend. The difference between the two groups was statistically significant (蠂 ~ 2 / 7.145 ~ (5) P 0.05 / L ~ (3)) in normal cervix. The positive expression rates of CINII-IIII and cervical cancer in the cervical intraepithelial neoplasia group and cervical cancer group were respectively:: 95.00.56.7and 38.0, and the expression was down-regulated. The difference between the two groups was statistically significant (蠂 ~ (2 / 2) 18.801 ~ (18.801)) (P 0.05), which indicated that ARHI LC3 was associated with the occurrence of cervical cancer. The expression level of ARHILC3 was related to the clinical stage and the degree of tissue differentiation. The difference was statistically significant (P 0.05), but the expression level of ARHILC3 was correlated with lymph node metastasis. There was no significant correlation between age and SCC. There was a positive correlation between the expression of ARHILC3 and the expression of ARHILC3 in cervical carcinoma. There were 68 cases with positive HPV infection in the above 100 cases. According to the degree of HPV infection, 68 cases were divided into 3 groups. With the severity of HPV infection, the expression level of ARHILC3 decreased, but the difference of LC3 in the three groups was statistically significant (蠂 ~ (2) 10.583) P 0.05, which indicated that the expression of LC3 was closely related to the degree of HPV infection, but there was a significant difference between the three groups (蠂 ~ (2 +) 10.583), indicating that the expression of LC3 was closely related to the degree of HPV infection. However, there was no significant difference between the expression level of ARHI and the degree of HPV infection. Conclusion the decrease and deletion of the expression level of ARHI LC3 may be involved in the occurrence and development of cervical cancer, and the expression level of LC3 is related to the degree of HPV infection.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.33

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